Spinal Cord Hemisection Facilitates Aromatic L-Amino Acid Decarboxylase Cells to Produce Serotonin in the Subchronic but Not the Chronic Phase.
(2015) In Neural Plasticity 2015.- Abstract
- Neuromodulators, such as serotonin (5-hydroxytryptamine, 5-HT) and noradrenalin, play an essential role in regulating the motor and sensory functions in the spinal cord. We have previously shown that in the rat spinal cord the activity of aromatic L-amino acid decarboxylase (AADC) cells to produce 5-HT from its precursor (5-hydroxytryptophan, 5-HTP) is dramatically increased following complete spinal cord transection. In this study, we investigated whether a partial loss of 5-HT innervation could similarly increase AADC activity. Adult rats with spinal cord hemisected at thoracic level (T11/T12) were used with a postoperation interval at 5 days or 60 days. Using immunohistochemistry, first, we observed a significant reduction in the... (More)
- Neuromodulators, such as serotonin (5-hydroxytryptamine, 5-HT) and noradrenalin, play an essential role in regulating the motor and sensory functions in the spinal cord. We have previously shown that in the rat spinal cord the activity of aromatic L-amino acid decarboxylase (AADC) cells to produce 5-HT from its precursor (5-hydroxytryptophan, 5-HTP) is dramatically increased following complete spinal cord transection. In this study, we investigated whether a partial loss of 5-HT innervation could similarly increase AADC activity. Adult rats with spinal cord hemisected at thoracic level (T11/T12) were used with a postoperation interval at 5 days or 60 days. Using immunohistochemistry, first, we observed a significant reduction in the density of 5-HT-immunoreactive fibers in the spinal cord below the lesion on the injured side for both groups. Second, we found that the AADC cells were similarly expressed on both injured and uninjured sides in both groups. Third, increased production of 5-HT in AADC cells following 5-HTP was seen in 5-day but not in 60-day postinjury group. These results suggest that plastic changes of the 5-HT system might happen primarily in the subchronic phase and for longer period its function could be compensated by plastic changes of other intrinsic and/or supraspinal modulation systems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8148240
- author
- Azam, Bushra ; Wienecke, Jacob ; Jensen, Dennis Bo ; Azam, Aleena and Zhang, Mengliang LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Neural Plasticity
- volume
- 2015
- article number
- 549671
- publisher
- Hindawi Limited
- external identifiers
-
- pmid:26504602
- wos:000363159000001
- scopus:84945279288
- pmid:26504602
- ISSN
- 2090-5904
- DOI
- 10.1155/2015/549671
- language
- English
- LU publication?
- yes
- id
- 0567a1fa-6238-4687-b142-a2f9d1eb5a68 (old id 8148240)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26504602?dopt=Abstract
- date added to LUP
- 2016-04-01 13:25:17
- date last changed
- 2022-01-27 19:08:33
@article{0567a1fa-6238-4687-b142-a2f9d1eb5a68, abstract = {{Neuromodulators, such as serotonin (5-hydroxytryptamine, 5-HT) and noradrenalin, play an essential role in regulating the motor and sensory functions in the spinal cord. We have previously shown that in the rat spinal cord the activity of aromatic L-amino acid decarboxylase (AADC) cells to produce 5-HT from its precursor (5-hydroxytryptophan, 5-HTP) is dramatically increased following complete spinal cord transection. In this study, we investigated whether a partial loss of 5-HT innervation could similarly increase AADC activity. Adult rats with spinal cord hemisected at thoracic level (T11/T12) were used with a postoperation interval at 5 days or 60 days. Using immunohistochemistry, first, we observed a significant reduction in the density of 5-HT-immunoreactive fibers in the spinal cord below the lesion on the injured side for both groups. Second, we found that the AADC cells were similarly expressed on both injured and uninjured sides in both groups. Third, increased production of 5-HT in AADC cells following 5-HTP was seen in 5-day but not in 60-day postinjury group. These results suggest that plastic changes of the 5-HT system might happen primarily in the subchronic phase and for longer period its function could be compensated by plastic changes of other intrinsic and/or supraspinal modulation systems.}}, author = {{Azam, Bushra and Wienecke, Jacob and Jensen, Dennis Bo and Azam, Aleena and Zhang, Mengliang}}, issn = {{2090-5904}}, language = {{eng}}, publisher = {{Hindawi Limited}}, series = {{Neural Plasticity}}, title = {{Spinal Cord Hemisection Facilitates Aromatic L-Amino Acid Decarboxylase Cells to Produce Serotonin in the Subchronic but Not the Chronic Phase.}}, url = {{https://lup.lub.lu.se/search/files/3359620/8840785}}, doi = {{10.1155/2015/549671}}, volume = {{2015}}, year = {{2015}}, }