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Gene expression profiling to define the cell intrinsic role of the SKI proto-oncogene in hematopoiesis and myeloid neoplasms.

Chalk, Alistair M; Liddicoat, Brian J J; Walkley, Carl R and Singbrant, Sofie LU (2014) In Genomics Data 2. p.189-191
Abstract
The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. However, its operative relevance in these cells remains elusive. We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. Accordingly, enforced expression of SKI induced gene signatures associated with hematopoietic stem cells and myeloid differentiation. Here we provide detailed experimental methods and analysis for the gene expression profiling described in our recently... (More)
The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. However, its operative relevance in these cells remains elusive. We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. Accordingly, enforced expression of SKI induced gene signatures associated with hematopoietic stem cells and myeloid differentiation. Here we provide detailed experimental methods and analysis for the gene expression profiling described in our recently published study of Singbrant et al. (2014) in Haematologica. Our data sets (available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39457) provide a resource for exploring the underlying molecular mechanisms of the involvement of the proto-oncogene SKI in hematopoietic stem cell function and development of myeloid neoplasms. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CDNA microarray, Gene expression profiling, Hematopoietic stem cell activity, Myeloproliferative disease, SKI
in
Genomics Data
volume
2
pages
189 - 191
publisher
Elsevier Inc.
external identifiers
  • pmid:26484093
  • scopus:84920568072
ISSN
2213-5960
DOI
10.1016/j.gdata.2014.06.022
language
English
LU publication?
yes
id
1b931fd8-fdcf-4338-9634-048975577f5f (old id 8148802)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26484093?dopt=Abstract
date added to LUP
2016-02-02 13:09:10
date last changed
2017-03-23 08:54:07
@article{1b931fd8-fdcf-4338-9634-048975577f5f,
  abstract     = {The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. However, its operative relevance in these cells remains elusive. We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. Accordingly, enforced expression of SKI induced gene signatures associated with hematopoietic stem cells and myeloid differentiation. Here we provide detailed experimental methods and analysis for the gene expression profiling described in our recently published study of Singbrant et al. (2014) in Haematologica. Our data sets (available at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39457) provide a resource for exploring the underlying molecular mechanisms of the involvement of the proto-oncogene SKI in hematopoietic stem cell function and development of myeloid neoplasms.},
  author       = {Chalk, Alistair M and Liddicoat, Brian J J and Walkley, Carl R and Singbrant, Sofie},
  issn         = {2213-5960},
  keyword      = {CDNA microarray,Gene expression profiling,Hematopoietic stem cell activity,Myeloproliferative disease,SKI},
  language     = {eng},
  pages        = {189--191},
  publisher    = {Elsevier Inc.},
  series       = {Genomics Data},
  title        = {Gene expression profiling to define the cell intrinsic role of the SKI proto-oncogene in hematopoiesis and myeloid neoplasms.},
  url          = {http://dx.doi.org/10.1016/j.gdata.2014.06.022},
  volume       = {2},
  year         = {2014},
}