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Temperate phages promote colicin-dependent fitness of Salmonella enterica serovar Typhimurium.

Nedialkova, Lubov Petkova; Sidstedt, Maja LU ; Koeppel, Martin B; Spriewald, Stefanie; Ring, Diana; Gerlach, Roman G; Bossi, Lionello and Stecher, Bärbel (2015) In Environmental Microbiology 18. p.1591-1603
Abstract
Bacteria employ bacteriocins for interference competition in microbial ecosystems. Colicin Ib (ColIb), a pore-forming bacteriocin, confers a significant fitness benefit to Salmonella enterica serovar Typhimurium (S. Tm) in competition against commensal E. coli in the gut. ColIb is released from S. Tm into the environment, where it kills susceptible competitors. However, colicin-specific release proteins, as they are known for other colicins, have not been identified in case of ColIb. Thus, its release mechanism has remained unclear. In the current study, we have established a new link between ColIb release and lysis activity of temperate, lambdoid phages. By the use of phage-cured S. Tm mutant strains, we show that the presence of... (More)
Bacteria employ bacteriocins for interference competition in microbial ecosystems. Colicin Ib (ColIb), a pore-forming bacteriocin, confers a significant fitness benefit to Salmonella enterica serovar Typhimurium (S. Tm) in competition against commensal E. coli in the gut. ColIb is released from S. Tm into the environment, where it kills susceptible competitors. However, colicin-specific release proteins, as they are known for other colicins, have not been identified in case of ColIb. Thus, its release mechanism has remained unclear. In the current study, we have established a new link between ColIb release and lysis activity of temperate, lambdoid phages. By the use of phage-cured S. Tm mutant strains, we show that the presence of temperate phages and their lysis genes is necessary and sufficient for release of active ColIb into the culture supernatant. Furthermore, phage-mediated lysis significantly enhanced S. Tm fitness in competition against a ColIb-susceptible competitor. Finally, transduction with the lambdoid phage 933W rescued the defect of E. coli strain MG1655 with respect to ColIb release. In conclusion, ColIb is released from bacteria in the course of phage lysis. Our data reveal a new mechanism for colicin release and point out a novel function of temperate phages in enhancing colicin-dependent bacterial fitness. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Microbiology
volume
18
pages
13 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:26439675
  • scopus:84971642824
ISSN
1462-2920
DOI
10.1111/1462-2920.13077
language
English
LU publication?
yes
id
a5c2781a-6495-40b0-aeaa-f02c8aa594c7 (old id 8158875)
date added to LUP
2015-11-15 22:49:55
date last changed
2017-10-22 03:12:12
@article{a5c2781a-6495-40b0-aeaa-f02c8aa594c7,
  abstract     = {Bacteria employ bacteriocins for interference competition in microbial ecosystems. Colicin Ib (ColIb), a pore-forming bacteriocin, confers a significant fitness benefit to Salmonella enterica serovar Typhimurium (S. Tm) in competition against commensal E. coli in the gut. ColIb is released from S. Tm into the environment, where it kills susceptible competitors. However, colicin-specific release proteins, as they are known for other colicins, have not been identified in case of ColIb. Thus, its release mechanism has remained unclear. In the current study, we have established a new link between ColIb release and lysis activity of temperate, lambdoid phages. By the use of phage-cured S. Tm mutant strains, we show that the presence of temperate phages and their lysis genes is necessary and sufficient for release of active ColIb into the culture supernatant. Furthermore, phage-mediated lysis significantly enhanced S. Tm fitness in competition against a ColIb-susceptible competitor. Finally, transduction with the lambdoid phage 933W rescued the defect of E. coli strain MG1655 with respect to ColIb release. In conclusion, ColIb is released from bacteria in the course of phage lysis. Our data reveal a new mechanism for colicin release and point out a novel function of temperate phages in enhancing colicin-dependent bacterial fitness.},
  author       = {Nedialkova, Lubov Petkova and Sidstedt, Maja and Koeppel, Martin B and Spriewald, Stefanie and Ring, Diana and Gerlach, Roman G and Bossi, Lionello and Stecher, Bärbel},
  issn         = {1462-2920},
  language     = {eng},
  month        = {10},
  pages        = {1591--1603},
  publisher    = {Wiley-Blackwell},
  series       = {Environmental Microbiology},
  title        = {Temperate phages promote colicin-dependent fitness of Salmonella enterica serovar Typhimurium.},
  url          = {http://dx.doi.org/10.1111/1462-2920.13077},
  volume       = {18},
  year         = {2015},
}