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Controlled Lateral Positioning of Microparticles Inside Droplets Using Acoustophoresis.

Fornell, Anna LU ; Nilsson, Johan LU ; Jonsson, Linus LU ; Periyannan Rajeswari, Prem Kumar; Jonsson, Haakan N and Tenje, Maria LU (2015) In Analytical Chemistry 87(20). p.10521-10526
Abstract
In this paper, we utilize bulk acoustic waves to control the position of microparticles inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the microparticles. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of microparticles during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated microparticles to the center of the droplets. The method is generic, requires no labeling of the microparticles, and is operated in a noncontact fashion. It was possible to achieve... (More)
In this paper, we utilize bulk acoustic waves to control the position of microparticles inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the microparticles. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of microparticles during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated microparticles to the center of the droplets. The method is generic, requires no labeling of the microparticles, and is operated in a noncontact fashion. It was possible to achieve 2+-fold enrichment of polystyrene beads (5 μm in diameter) in the center daughter droplet with an average recovery of 89% of the beads. Red blood cells were also successfully manipulated inside droplets. These results show the possibility to use acoustophoresis in two-phase systems to enrich microparticles and open up the possibility for new droplet-based assays that are not performed today. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
87
issue
20
pages
10521 - 10526
publisher
The American Chemical Society
external identifiers
  • pmid:26422760
  • wos:000363348300051
  • scopus:84945288371
ISSN
1520-6882
DOI
10.1021/acs.analchem.5b02746
language
English
LU publication?
yes
id
d63f1cca-251e-4d97-b197-3307868324df (old id 8159922)
date added to LUP
2015-11-26 11:02:32
date last changed
2017-11-12 03:08:13
@article{d63f1cca-251e-4d97-b197-3307868324df,
  abstract     = {In this paper, we utilize bulk acoustic waves to control the position of microparticles inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the microparticles. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of microparticles during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated microparticles to the center of the droplets. The method is generic, requires no labeling of the microparticles, and is operated in a noncontact fashion. It was possible to achieve 2+-fold enrichment of polystyrene beads (5 μm in diameter) in the center daughter droplet with an average recovery of 89% of the beads. Red blood cells were also successfully manipulated inside droplets. These results show the possibility to use acoustophoresis in two-phase systems to enrich microparticles and open up the possibility for new droplet-based assays that are not performed today.},
  author       = {Fornell, Anna and Nilsson, Johan and Jonsson, Linus and Periyannan Rajeswari, Prem Kumar and Jonsson, Haakan N and Tenje, Maria},
  issn         = {1520-6882},
  language     = {eng},
  number       = {20},
  pages        = {10521--10526},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Controlled Lateral Positioning of Microparticles Inside Droplets Using Acoustophoresis.},
  url          = {http://dx.doi.org/10.1021/acs.analchem.5b02746},
  volume       = {87},
  year         = {2015},
}