Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude
(2006) In Analyst 131(7). p.822-828- Abstract
- We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or... (More)
- We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or activate - binding reactions in a controlled way, while simultaneously maintaining the possibility of probing the adsorption and/or desorption events in a non-perturbative manner using combined f and D measurements. In this work we investigate the influence of oscillation amplitude variations on the binding of NeutrAvidin-modified polystyrene beads (O similar to 200 nm) to a planar biotin-modified lipid bilayer supported on an SiO2-modified QCM-D sensor. These results are further compared with data on an identical system, except that the NeutrAvidin-biotin recognition was replaced by fully complementary DNA hybridization. Supported by micrographs of the binding pattern, the results demonstrate that there exists, for both systems, a unique critical oscillation amplitude, A(c), below which binding is unaffected by the oscillation, and above which binding is efficiently prevented. Associated with A(c), there is a critical crystal radius, r(c), defining the central part of the crystal where binding is prevented. From QCM-D data, A(c) for the present system was estimated to be similar to 6.5 nm, yielding a value of r(c) of similar to 3 mm - the latter number was nicely confirmed by fluorescent- and dark-field micrographs of the crystal. Furthermore, the fact that A(c) is observed to be identical for the two types of biorecognition reactions suggests that it is neither the strength, nor the number of contact points, that determine the amplitude at which binding is prevented. Rather, particle size seems to be the determining parameter. (Less)
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https://lup.lub.lu.se/record/405784
- author
- Edvardsson, M ; Rodahl, M and Höök, Fredrik LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analyst
- volume
- 131
- issue
- 7
- pages
- 822 - 828
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000238560900009
- scopus:33745496747
- ISSN
- 1364-5528
- DOI
- 10.1039/b601800a
- language
- English
- LU publication?
- yes
- id
- 81621072-a6a5-4c8a-b514-3c8267cc6617 (old id 405784)
- date added to LUP
- 2016-04-01 16:05:13
- date last changed
- 2022-02-12 19:42:40
@article{81621072-a6a5-4c8a-b514-3c8267cc6617,
abstract = {{We report measurements with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, with focus on how the shear oscillation amplitude of the sensor surface influences biorecognition binding events. Technically, this is made as reported recently ( M. Edvardsson, M. Rodahl, B. Kasemo, F. Hook, Anal. Chem., 2005, 77( 15), 4918-4926) by operating the QCM in dual frequency mode; one harmonic (n = n(1)) is utilized for continuous excitation of the QCM-D sensor at resonance at variable driving amplitudes (1-10 V), while the second harmonic (n not equal n(1)) is used for combined f and D measurements. By using one harmonic as a "probe" and the other one as an "actuator", elevated amplitudes can be used to perturb - or activate - binding reactions in a controlled way, while simultaneously maintaining the possibility of probing the adsorption and/or desorption events in a non-perturbative manner using combined f and D measurements. In this work we investigate the influence of oscillation amplitude variations on the binding of NeutrAvidin-modified polystyrene beads (O similar to 200 nm) to a planar biotin-modified lipid bilayer supported on an SiO2-modified QCM-D sensor. These results are further compared with data on an identical system, except that the NeutrAvidin-biotin recognition was replaced by fully complementary DNA hybridization. Supported by micrographs of the binding pattern, the results demonstrate that there exists, for both systems, a unique critical oscillation amplitude, A(c), below which binding is unaffected by the oscillation, and above which binding is efficiently prevented. Associated with A(c), there is a critical crystal radius, r(c), defining the central part of the crystal where binding is prevented. From QCM-D data, A(c) for the present system was estimated to be similar to 6.5 nm, yielding a value of r(c) of similar to 3 mm - the latter number was nicely confirmed by fluorescent- and dark-field micrographs of the crystal. Furthermore, the fact that A(c) is observed to be identical for the two types of biorecognition reactions suggests that it is neither the strength, nor the number of contact points, that determine the amplitude at which binding is prevented. Rather, particle size seems to be the determining parameter.}},
author = {{Edvardsson, M and Rodahl, M and Höök, Fredrik}},
issn = {{1364-5528}},
language = {{eng}},
number = {{7}},
pages = {{822--828}},
publisher = {{Royal Society of Chemistry}},
series = {{Analyst}},
title = {{Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude}},
url = {{http://dx.doi.org/10.1039/b601800a}},
doi = {{10.1039/b601800a}},
volume = {{131}},
year = {{2006}},
}