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Deletion of the Bacillus subtilis sdh operon

Fridén, H; Hederstedt, Lars LU and Rutberg, Lars (1987) In FEMS Microbiology Letters 41(2). p.206-206
Abstract
Plasmid pKIM2 carries the Bacillus subtilis sdh operon and adjacent regions of the bacterial chromosome. The plasmid replicates in Escherichia coli but not in B. subtilis. Different portions of the sdh operon were removed from pKIM2 and replaced by a cat gene derived from pC194. A series of plasmids carrying sdh deletions was thus derived. Plasmid DNA was linearized at restriction sites within the vector part and used to transform B. subtilis to chloramphenicol resistance. The majority of the transformants had a succinate dehydrogenase-negative phenotype and were deleted in the sdh operon as verified by Southern blotting. The B. subtilis deletion mutants were used to determine the functional integrity of cloned sdh genes.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Succinate dehydrogenase
in
FEMS Microbiology Letters
volume
41
issue
2
pages
206 - 206
publisher
Elsevier
ISSN
1574-6968
DOI
10.1111/j.1574-6968.1987.tb02197.x
language
English
LU publication?
yes
id
817a5e5b-e8e1-4c0d-ba83-3d3edfd53178
date added to LUP
2017-07-18 11:01:36
date last changed
2017-09-13 16:37:12
@article{817a5e5b-e8e1-4c0d-ba83-3d3edfd53178,
  abstract     = {Plasmid pKIM2 carries the Bacillus subtilis sdh operon and adjacent regions of the bacterial chromosome. The plasmid replicates in Escherichia coli but not in B. subtilis. Different portions of the sdh operon were removed from pKIM2 and replaced by a cat gene derived from pC194. A series of plasmids carrying sdh deletions was thus derived. Plasmid DNA was linearized at restriction sites within the vector part and used to transform B. subtilis to chloramphenicol resistance. The majority of the transformants had a succinate dehydrogenase-negative phenotype and were deleted in the sdh operon as verified by Southern blotting. The B. subtilis deletion mutants were used to determine the functional integrity of cloned sdh genes.},
  author       = {Fridén, H and Hederstedt, Lars and Rutberg, Lars},
  issn         = {1574-6968},
  keyword      = {Succinate dehydrogenase},
  language     = {eng},
  number       = {2},
  pages        = {206--206},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Deletion of the <em>Bacillus subtilis sdh</em> operon},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.1987.tb02197.x},
  volume       = {41},
  year         = {1987},
}