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Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase

Zhang, M ; Aman, Pierre LU ; Grubb, Anders LU orcid ; Panagopoulos, Ioannis LU ; Hindemith, A ; Rosengren, Evald LU and Rorsman, Hans LU (1995) In FEBS Letters 373(3). p.203-206
Abstract

An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on... (More)

An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat D-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Cloning, Molecular, DNA Primers, DNA, Complementary, Humans, Indolequinones, Indoles/metabolism, Intramolecular Oxidoreductases, Isomerases/chemistry, Liver/enzymology, Macrophage Migration-Inhibitory Factors/chemistry, Molecular Sequence Data, Open Reading Frames/genetics, Quinones/metabolism, Rats, Sequence Homology, Nucleic Acid
in
FEBS Letters
volume
373
issue
3
pages
203 - 206
publisher
Wiley-Blackwell
external identifiers
  • pmid:7589466
  • scopus:0028874828
ISSN
0014-5793
DOI
10.1016/0014-5793(95)01041-c
language
English
LU publication?
no
id
819fa342-5e22-4ea4-97c2-b13c8c96e8ea
date added to LUP
2021-10-28 14:48:49
date last changed
2024-01-12 02:55:38
@article{819fa342-5e22-4ea4-97c2-b13c8c96e8ea,
  abstract     = {{<p>An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat D-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).</p>}},
  author       = {{Zhang, M and Aman, Pierre and Grubb, Anders and Panagopoulos, Ioannis and Hindemith, A and Rosengren, Evald and Rorsman, Hans}},
  issn         = {{0014-5793}},
  keywords     = {{Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA Primers; DNA, Complementary; Humans; Indolequinones; Indoles/metabolism; Intramolecular Oxidoreductases; Isomerases/chemistry; Liver/enzymology; Macrophage Migration-Inhibitory Factors/chemistry; Molecular Sequence Data; Open Reading Frames/genetics; Quinones/metabolism; Rats; Sequence Homology, Nucleic Acid}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{203--206}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase}},
  url          = {{http://dx.doi.org/10.1016/0014-5793(95)01041-c}},
  doi          = {{10.1016/0014-5793(95)01041-c}},
  volume       = {{373}},
  year         = {{1995}},
}