Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase
(1995) In FEBS Letters 373(3). p.203-206- Abstract
An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on... (More)
An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat D-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).
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- author
- Zhang, M ; Aman, Pierre LU ; Grubb, Anders LU ; Panagopoulos, Ioannis LU ; Hindemith, A ; Rosengren, Evald LU and Rorsman, Hans LU
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- keywords
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Cloning, Molecular, DNA Primers, DNA, Complementary, Humans, Indolequinones, Indoles/metabolism, Intramolecular Oxidoreductases, Isomerases/chemistry, Liver/enzymology, Macrophage Migration-Inhibitory Factors/chemistry, Molecular Sequence Data, Open Reading Frames/genetics, Quinones/metabolism, Rats, Sequence Homology, Nucleic Acid
- in
- FEBS Letters
- volume
- 373
- issue
- 3
- pages
- 203 - 206
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028874828
- pmid:7589466
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(95)01041-c
- language
- English
- LU publication?
- no
- id
- 819fa342-5e22-4ea4-97c2-b13c8c96e8ea
- date added to LUP
- 2021-10-28 14:48:49
- date last changed
- 2024-01-12 02:55:38
@article{819fa342-5e22-4ea4-97c2-b13c8c96e8ea, abstract = {{<p>An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat D-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).</p>}}, author = {{Zhang, M and Aman, Pierre and Grubb, Anders and Panagopoulos, Ioannis and Hindemith, A and Rosengren, Evald and Rorsman, Hans}}, issn = {{0014-5793}}, keywords = {{Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA Primers; DNA, Complementary; Humans; Indolequinones; Indoles/metabolism; Intramolecular Oxidoreductases; Isomerases/chemistry; Liver/enzymology; Macrophage Migration-Inhibitory Factors/chemistry; Molecular Sequence Data; Open Reading Frames/genetics; Quinones/metabolism; Rats; Sequence Homology, Nucleic Acid}}, language = {{eng}}, number = {{3}}, pages = {{203--206}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase}}, url = {{http://dx.doi.org/10.1016/0014-5793(95)01041-c}}, doi = {{10.1016/0014-5793(95)01041-c}}, volume = {{373}}, year = {{1995}}, }