Time domain diffuse Raman spectroscopy using single pixel detection
(2023) In Biomedical Optics Express 14(11). p.5749-5763- Abstract
- Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the... (More)
- Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the system using a bilayer tissue-bone mimicking phantom composed of a 1 cm thick slab of silicone overlaying a calcium carbonate specimen and demonstrated a high differentiation of the two Raman signals. We reconstructed the Raman spectra of the two layers, offering the potential for improved and quantitative material analysis. Using a bilayer phantom made of porcine muscle and calcium carbonate, we proved that our system can retrieve Raman peaks even in the presence of autofluorescence typical of biomedical tissues. Overall, our novel TD-DIRS setup proposes a cost-effective and high-performance approach for in-depth Raman spectroscopy in diffusive media. (Less)
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https://lup.lub.lu.se/record/81cd11a4-920b-417e-8d58-84a00686708e
- author
- organization
- publishing date
- 2023-10-13
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomedical Optics Express
- volume
- 14
- issue
- 11
- pages
- 5749 - 5763
- publisher
- Optical Society of America
- external identifiers
-
- scopus:85176274681
- pmid:38021118
- ISSN
- 2156-7085
- DOI
- 10.1364/BOE.502022
- language
- English
- LU publication?
- yes
- id
- 81cd11a4-920b-417e-8d58-84a00686708e
- date added to LUP
- 2023-11-16 14:43:51
- date last changed
- 2024-02-16 03:00:48
@article{81cd11a4-920b-417e-8d58-84a00686708e, abstract = {{Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the system using a bilayer tissue-bone mimicking phantom composed of a 1 cm thick slab of silicone overlaying a calcium carbonate specimen and demonstrated a high differentiation of the two Raman signals. We reconstructed the Raman spectra of the two layers, offering the potential for improved and quantitative material analysis. Using a bilayer phantom made of porcine muscle and calcium carbonate, we proved that our system can retrieve Raman peaks even in the presence of autofluorescence typical of biomedical tissues. Overall, our novel TD-DIRS setup proposes a cost-effective and high-performance approach for in-depth Raman spectroscopy in diffusive media.}}, author = {{Bossi, Alessandro and Sekar, Sanathana Konugolu Venkata and Lacerenza, Michele and Gandolfi, Valerio and Susnjar, Stefan and Lanka, Pranav and D'Andrea, Cosimo and Vanna, Renzo and Valentini, Gianluca and Farina, Andrea and Pifferi, Antonio}}, issn = {{2156-7085}}, language = {{eng}}, month = {{10}}, number = {{11}}, pages = {{5749--5763}}, publisher = {{Optical Society of America}}, series = {{Biomedical Optics Express}}, title = {{Time domain diffuse Raman spectroscopy using single pixel detection}}, url = {{http://dx.doi.org/10.1364/BOE.502022}}, doi = {{10.1364/BOE.502022}}, volume = {{14}}, year = {{2023}}, }