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Time domain diffuse Raman spectroscopy using single pixel detection

Bossi, Alessandro ; Sekar, Sanathana Konugolu Venkata ; Lacerenza, Michele ; Gandolfi, Valerio ; Susnjar, Stefan LU ; Lanka, Pranav ; D'Andrea, Cosimo ; Vanna, Renzo ; Valentini, Gianluca and Farina, Andrea , et al. (2023) In Biomedical Optics Express 14(11). p.5749-5763
Abstract
Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the... (More)
Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the system using a bilayer tissue-bone mimicking phantom composed of a 1 cm thick slab of silicone overlaying a calcium carbonate specimen and demonstrated a high differentiation of the two Raman signals. We reconstructed the Raman spectra of the two layers, offering the potential for improved and quantitative material analysis. Using a bilayer phantom made of porcine muscle and calcium carbonate, we proved that our system can retrieve Raman peaks even in the presence of autofluorescence typical of biomedical tissues. Overall, our novel TD-DIRS setup proposes a cost-effective and high-performance approach for in-depth Raman spectroscopy in diffusive media. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedical Optics Express
volume
14
issue
11
pages
5749 - 5763
publisher
Optical Society of America
external identifiers
  • scopus:85176274681
  • pmid:38021118
ISSN
2156-7085
DOI
10.1364/BOE.502022
language
English
LU publication?
yes
id
81cd11a4-920b-417e-8d58-84a00686708e
date added to LUP
2023-11-16 14:43:51
date last changed
2024-02-16 03:00:48
@article{81cd11a4-920b-417e-8d58-84a00686708e,
  abstract     = {{Diffuse Raman spectroscopy (DIRS) extends the high chemical specificity of Raman scattering to in-depth investigation of thick biological tissues. We present here a novel approach for time-domain diffuse Raman spectroscopy (TD-DIRS) based on a single-pixel detector and a digital micromirror device (DMD) within an imaging spectrometer for wavelength encoding. This overcomes the intrinsic complexity and high cost of detection arrays with ps-resolving time capability. Unlike spatially offset Raman spectroscopy (SORS) or frequency offset Raman spectroscopy (FORS), TD-DIRS exploits the time-of-flight distribution of photons to probe the depth of the Raman signal at a single wavelength with a single source-detector separation. We validated the system using a bilayer tissue-bone mimicking phantom composed of a 1 cm thick slab of silicone overlaying a calcium carbonate specimen and demonstrated a high differentiation of the two Raman signals. We reconstructed the Raman spectra of the two layers, offering the potential for improved and quantitative material analysis. Using a bilayer phantom made of porcine muscle and calcium carbonate, we proved that our system can retrieve Raman peaks even in the presence of autofluorescence typical of biomedical tissues. Overall, our novel TD-DIRS setup proposes a cost-effective and high-performance approach for in-depth Raman spectroscopy in diffusive media.}},
  author       = {{Bossi, Alessandro and Sekar, Sanathana Konugolu Venkata and Lacerenza, Michele and Gandolfi, Valerio and Susnjar, Stefan and Lanka, Pranav and D'Andrea, Cosimo and Vanna, Renzo and Valentini, Gianluca and Farina, Andrea and Pifferi, Antonio}},
  issn         = {{2156-7085}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{11}},
  pages        = {{5749--5763}},
  publisher    = {{Optical Society of America}},
  series       = {{Biomedical Optics Express}},
  title        = {{Time domain diffuse Raman spectroscopy using single pixel detection}},
  url          = {{http://dx.doi.org/10.1364/BOE.502022}},
  doi          = {{10.1364/BOE.502022}},
  volume       = {{14}},
  year         = {{2023}},
}