Effects of compounds from physalis angulata on fatty acid synthesis and glucose metabolism in HEPG2 cells via the AMP-activated protein kinase pathway
(2020) In Natural Product Sciences 26(3). p.200-206- Abstract
The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 – 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 μg/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 – 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC.... (More)
The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 – 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 μg/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 – 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 – 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism.
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- author
- Hoa, Hoang Thai ; Thu, Nguyen Thi ; Dong, Nguyen Thuong ; Oanh, Tran Thi ; Hien, Tran Thi LU and Ha, Do Thi
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ACC, AMPK, FAS, Physalis angulata, SREBP-1c, Withanolides
- in
- Natural Product Sciences
- volume
- 26
- issue
- 3
- pages
- 7 pages
- publisher
- Korean Society of Pharmacognosy
- external identifiers
-
- scopus:85096587225
- ISSN
- 1226-3907
- DOI
- 10.20307/nps.2020.26.3.200
- language
- English
- LU publication?
- yes
- id
- 81e9409e-8131-415b-8564-ea6ecd3d1521
- date added to LUP
- 2020-12-08 14:28:19
- date last changed
- 2022-04-19 02:29:56
@article{81e9409e-8131-415b-8564-ea6ecd3d1521,
abstract = {{<p>The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 – 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 μg/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 – 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 – 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism.</p>}},
author = {{Hoa, Hoang Thai and Thu, Nguyen Thi and Dong, Nguyen Thuong and Oanh, Tran Thi and Hien, Tran Thi and Ha, Do Thi}},
issn = {{1226-3907}},
keywords = {{ACC; AMPK; FAS; Physalis angulata; SREBP-1c; Withanolides}},
language = {{eng}},
number = {{3}},
pages = {{200--206}},
publisher = {{Korean Society of Pharmacognosy}},
series = {{Natural Product Sciences}},
title = {{Effects of compounds from physalis angulata on fatty acid synthesis and glucose metabolism in HEPG2 cells via the AMP-activated protein kinase pathway}},
url = {{http://dx.doi.org/10.20307/nps.2020.26.3.200}},
doi = {{10.20307/nps.2020.26.3.200}},
volume = {{26}},
year = {{2020}},
}