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Characterisation of starch: development of analytical methods

Nilsson, Gunilla LU (1999)
Abstract
Starch, comprised of amylose and amylopectin, is a very complex biopolymer. Different methods are therefore needed for the investigation of the molecular structures of amylose and amylopectin. In this thesis analytical methods have been developed and various techniques were combined for the characterisation of starch. Microdialysis was used for sampling and sample clean-up coupled on-line with anion-exchange chromatography for the determination of starch hydrolysis products. Both enzyme-based hydrolysis of native and modified starch and enzyme-based detection systems were studied. The enzymes were used either in solution or immobilised in enzyme reactors or in a biosensor format. Molecular parameters for native starch, amylose and... (More)
Starch, comprised of amylose and amylopectin, is a very complex biopolymer. Different methods are therefore needed for the investigation of the molecular structures of amylose and amylopectin. In this thesis analytical methods have been developed and various techniques were combined for the characterisation of starch. Microdialysis was used for sampling and sample clean-up coupled on-line with anion-exchange chromatography for the determination of starch hydrolysis products. Both enzyme-based hydrolysis of native and modified starch and enzyme-based detection systems were studied. The enzymes were used either in solution or immobilised in enzyme reactors or in a biosensor format. Molecular parameters for native starch, amylose and amylopectin were determined such as the degree of branching, blue-value, chain length distribution, and b-limit value. The substitution patterns of modified starch derivatised under homogenous or heterogeneous conditions were determined. Key-criteria in the development of analytical methods are selectivity, sensitivity, repeatability, accuracy, limit of detection, simplicity, total analysis time, and automatisation. The last three criteria were addressed by using on-line and flow injection techniques. Selectivity was obtained by applying enzyme-based methods. The variations in the results obtained with various methods were discussed in the summary of the eight papers included in this thesis. (Less)
Abstract (Swedish)
Popular Abstract in Swedish

Karaktärisering av stärkelse: utveckling av analytiska metoder
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Docent Bertoft, Eric
organization
publishing date
type
Thesis
publication status
published
subject
keywords
substitution pattern, microdialysis, HPAEC-PAD, NMR, biosensor, IMER, potato amylopectin starch, amylose, amylopectin, chain length, Analytical chemistry, Analytisk kemi
pages
180 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Chemical Centre, Lecture hall C
defense date
1999-05-25 14:15:00
external identifiers
  • other:ISRN: LUNKDL/NKAK-1052/1-180
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
81fbd4fd-13a5-4c40-b2d4-310f3ee2da0d (old id 39703)
date added to LUP
2016-04-04 11:13:08
date last changed
2018-11-21 21:03:25
@phdthesis{81fbd4fd-13a5-4c40-b2d4-310f3ee2da0d,
  abstract     = {Starch, comprised of amylose and amylopectin, is a very complex biopolymer. Different methods are therefore needed for the investigation of the molecular structures of amylose and amylopectin. In this thesis analytical methods have been developed and various techniques were combined for the characterisation of starch. Microdialysis was used for sampling and sample clean-up coupled on-line with anion-exchange chromatography for the determination of starch hydrolysis products. Both enzyme-based hydrolysis of native and modified starch and enzyme-based detection systems were studied. The enzymes were used either in solution or immobilised in enzyme reactors or in a biosensor format. Molecular parameters for native starch, amylose and amylopectin were determined such as the degree of branching, blue-value, chain length distribution, and b-limit value. The substitution patterns of modified starch derivatised under homogenous or heterogeneous conditions were determined. Key-criteria in the development of analytical methods are selectivity, sensitivity, repeatability, accuracy, limit of detection, simplicity, total analysis time, and automatisation. The last three criteria were addressed by using on-line and flow injection techniques. Selectivity was obtained by applying enzyme-based methods. The variations in the results obtained with various methods were discussed in the summary of the eight papers included in this thesis.},
  author       = {Nilsson, Gunilla},
  language     = {eng},
  publisher    = {Department of Analytical Chemistry, Lund University},
  school       = {Lund University},
  title        = {Characterisation of starch: development of analytical methods},
  year         = {1999},
}