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Technical Note: Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos - the calcein-osmotic pump method

Bernhard, J. M.; Phalen, W. G.; McIntyre-Wressnig, A.; Mezzo, F.; Wit, J. C.; Jeglinski, M. and Filipsson, Helena LU (2015) In Biogeosciences 12(18). p.5515-5522
Abstract
Insights into oceanographic environmental conditions such as paleoproductivity, deep-water temperatures, salinity, ice volumes, and nutrient cycling have all been obtained from geochemical analyses of biomineralized carbonate of marine organisms. However, we cannot fully understand geochemical proxy incorporation and the fidelity of such in species until we better understand fundamental aspects of their ecology such as where and when these (micro) organisms calcify. Here, we present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous meiofauna calcify in situ. Method development initially involved juvenile quahogs (Mercenaria mercenaria); subsequent method refinement... (More)
Insights into oceanographic environmental conditions such as paleoproductivity, deep-water temperatures, salinity, ice volumes, and nutrient cycling have all been obtained from geochemical analyses of biomineralized carbonate of marine organisms. However, we cannot fully understand geochemical proxy incorporation and the fidelity of such in species until we better understand fundamental aspects of their ecology such as where and when these (micro) organisms calcify. Here, we present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous meiofauna calcify in situ. Method development initially involved juvenile quahogs (Mercenaria mercenaria); subsequent method refinement involved a neritic benthic foraminiferal community. Future applications of this method will allow determining the in situ growth rate in calcareous organisms and provide insights about microhabitats where paleoceanographically relevant benthic foraminifera actually calcify. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biogeosciences
volume
12
issue
18
pages
5515 - 5522
publisher
Copernicus Publications
external identifiers
  • wos:000362208800003
  • scopus:84942802367
ISSN
1726-4189
DOI
10.5194/bg-12-5515-2015
language
English
LU publication?
yes
id
33d132c0-4e0d-4f3c-a2bc-d096adbed919 (old id 8227201)
date added to LUP
2015-12-02 08:36:57
date last changed
2017-01-01 03:41:27
@article{33d132c0-4e0d-4f3c-a2bc-d096adbed919,
  abstract     = {Insights into oceanographic environmental conditions such as paleoproductivity, deep-water temperatures, salinity, ice volumes, and nutrient cycling have all been obtained from geochemical analyses of biomineralized carbonate of marine organisms. However, we cannot fully understand geochemical proxy incorporation and the fidelity of such in species until we better understand fundamental aspects of their ecology such as where and when these (micro) organisms calcify. Here, we present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous meiofauna calcify in situ. Method development initially involved juvenile quahogs (Mercenaria mercenaria); subsequent method refinement involved a neritic benthic foraminiferal community. Future applications of this method will allow determining the in situ growth rate in calcareous organisms and provide insights about microhabitats where paleoceanographically relevant benthic foraminifera actually calcify.},
  author       = {Bernhard, J. M. and Phalen, W. G. and McIntyre-Wressnig, A. and Mezzo, F. and Wit, J. C. and Jeglinski, M. and Filipsson, Helena},
  issn         = {1726-4189},
  language     = {eng},
  number       = {18},
  pages        = {5515--5522},
  publisher    = {Copernicus Publications},
  series       = {Biogeosciences},
  title        = {Technical Note: Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos - the calcein-osmotic pump method},
  url          = {http://dx.doi.org/10.5194/bg-12-5515-2015},
  volume       = {12},
  year         = {2015},
}