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Electrochemical communication between heterotrophically grown Rhodobacter capsulatus with electrodes mediated by an osmium redox polymer.

Hasan, Kamrul LU ; Patil, Sunil LU ; Gorecki, Kamil LU ; Leech, Dónal; Hägerhäll, Cecilia LU and Gorton, Lo LU (2013) In Bioelectrochemistry 93(Online 15 June 2012). p.30-36
Abstract
The metabolically versatile purple bacteria Rhodobacter capsulatus was investigated to check its possible applicability in biofuel cells and electrochemical microbial biosensors. The wild type strain ATCC 17015 and mutant strain 37b4 lacking the lipopolysaccharide capsule was compared for their ability to communicate with electrodes modified with an osmium redox polymer. In this work, aerobic heterotrophically grown R. capsulatus were used to screen for efficient cell-electrode communication for later implementation using photoheterotrophically grown bacteria. The bacterial cells embedded in the osmium polymer matrix demonstrated efficient electrical "wiring" with the electrodes and were able to generate a noticeable current with succinate... (More)
The metabolically versatile purple bacteria Rhodobacter capsulatus was investigated to check its possible applicability in biofuel cells and electrochemical microbial biosensors. The wild type strain ATCC 17015 and mutant strain 37b4 lacking the lipopolysaccharide capsule was compared for their ability to communicate with electrodes modified with an osmium redox polymer. In this work, aerobic heterotrophically grown R. capsulatus were used to screen for efficient cell-electrode communication for later implementation using photoheterotrophically grown bacteria. The bacterial cells embedded in the osmium polymer matrix demonstrated efficient electrical "wiring" with the electrodes and were able to generate a noticeable current with succinate as substrate. Interestingly, at 2mM succinate the wild type strain showed much better bioelectrocatalytic current generation (4.25μA/cm(2)) than the strain lacking capsule (1.55μA/cm(2)). The wild type strain also exhibited a stable current response for longer time, demonstrating that the bacterial lipopolysaccharide in fact enhances the stability of the polymer matrix layer of the modified electrode. Control experiments with R. capsulatus without any mediator did not show any current irrespective of the capsule presence. This demonstrates that development of photosensors and other light driven bioelectrochemical devices could be feasible using R. capsulatus and will be at focus for future studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioelectrochemistry
volume
93
issue
Online 15 June 2012
pages
30 - 36
publisher
Elsevier
external identifiers
  • wos:000324453100005
  • pmid:22749669
  • scopus:84882850086
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2012.05.004
language
English
LU publication?
yes
id
823274a0-a0fd-4a3a-a642-503e7cd550fa (old id 2967625)
date added to LUP
2012-09-10 11:56:03
date last changed
2019-03-12 01:11:32
@article{823274a0-a0fd-4a3a-a642-503e7cd550fa,
  abstract     = {The metabolically versatile purple bacteria Rhodobacter capsulatus was investigated to check its possible applicability in biofuel cells and electrochemical microbial biosensors. The wild type strain ATCC 17015 and mutant strain 37b4 lacking the lipopolysaccharide capsule was compared for their ability to communicate with electrodes modified with an osmium redox polymer. In this work, aerobic heterotrophically grown R. capsulatus were used to screen for efficient cell-electrode communication for later implementation using photoheterotrophically grown bacteria. The bacterial cells embedded in the osmium polymer matrix demonstrated efficient electrical "wiring" with the electrodes and were able to generate a noticeable current with succinate as substrate. Interestingly, at 2mM succinate the wild type strain showed much better bioelectrocatalytic current generation (4.25μA/cm(2)) than the strain lacking capsule (1.55μA/cm(2)). The wild type strain also exhibited a stable current response for longer time, demonstrating that the bacterial lipopolysaccharide in fact enhances the stability of the polymer matrix layer of the modified electrode. Control experiments with R. capsulatus without any mediator did not show any current irrespective of the capsule presence. This demonstrates that development of photosensors and other light driven bioelectrochemical devices could be feasible using R. capsulatus and will be at focus for future studies.},
  author       = {Hasan, Kamrul and Patil, Sunil and Gorecki, Kamil and Leech, Dónal and Hägerhäll, Cecilia and Gorton, Lo},
  issn         = {1878-562X},
  language     = {eng},
  number       = {Online 15 June 2012},
  pages        = {30--36},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Electrochemical communication between heterotrophically grown Rhodobacter capsulatus with electrodes mediated by an osmium redox polymer.},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2012.05.004},
  volume       = {93},
  year         = {2013},
}