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Expression of Multiple Functional RNAs or Proteins from One Viral Vector.

Björklund, Tomas LU (2016) In Methods in Molecular Biology 1382. p.41-56
Abstract
In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1382
pages
41 - 56
publisher
Springer
external identifiers
  • pmid:26611577
  • scopus:84948844870
ISSN
1940-6029
DOI
10.1007/978-1-4939-3271-9_3
language
English
LU publication?
yes
id
4611f159-b36a-499d-a393-d74093e0e70b (old id 8234437)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26611577?dopt=Abstract
date added to LUP
2015-12-01 16:18:51
date last changed
2017-03-03 15:41:51
@article{4611f159-b36a-499d-a393-d74093e0e70b,
  abstract     = {In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.},
  author       = {Björklund, Tomas},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {41--56},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Expression of Multiple Functional RNAs or Proteins from One Viral Vector.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3271-9_3},
  volume       = {1382},
  year         = {2016},
}