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Study of in Vitro and in Vivo Bone Formation in Composite Cryogels and the Influence of Electrical Stimulation.

Mishra, Ruchi; Raina, Deepak LU ; Pelkonen, Mea LU ; Lidgren, Lars LU ; Tägil, Magnus LU and Kumar, Ashok (2015) In International Journal of Biological Sciences 11(11). p.1325-1336
Abstract
This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further,... (More)
This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further, osteogenic differentiation of murine mesenchymal stem cells was studied with and without electrical stimulation. The q-PCR analysis shows that the electrically stimulated cryogels exhibit ~ 6 folds higher collagen type I and ~ 10 folds higher osteopontin mRNA level, in comparison to the unstimulated cryogels. Thus, PTAC biocomposite cryogels present osteoinductive and osteoconductive properties during in vitro and in vivo studies and support osteogenic differentiation of mesenchymal stem cells under the influence of electrical stimulation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Biological Sciences
volume
11
issue
11
pages
1325 - 1336
publisher
Ivyspring International Publisher
external identifiers
  • pmid:26535027
  • wos:000363677500009
  • scopus:84945309162
ISSN
1449-2288
DOI
10.7150/ijbs.13139
language
English
LU publication?
yes
id
1ba1b8d7-c8f1-4421-b209-f9b1c3116782 (old id 8243177)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26535027?dopt=Abstract
date added to LUP
2015-12-02 22:40:50
date last changed
2017-11-19 04:02:58
@article{1ba1b8d7-c8f1-4421-b209-f9b1c3116782,
  abstract     = {This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further, osteogenic differentiation of murine mesenchymal stem cells was studied with and without electrical stimulation. The q-PCR analysis shows that the electrically stimulated cryogels exhibit ~ 6 folds higher collagen type I and ~ 10 folds higher osteopontin mRNA level, in comparison to the unstimulated cryogels. Thus, PTAC biocomposite cryogels present osteoinductive and osteoconductive properties during in vitro and in vivo studies and support osteogenic differentiation of mesenchymal stem cells under the influence of electrical stimulation.},
  author       = {Mishra, Ruchi and Raina, Deepak and Pelkonen, Mea and Lidgren, Lars and Tägil, Magnus and Kumar, Ashok},
  issn         = {1449-2288},
  language     = {eng},
  number       = {11},
  pages        = {1325--1336},
  publisher    = {Ivyspring International Publisher},
  series       = {International Journal of Biological Sciences},
  title        = {Study of in Vitro and in Vivo Bone Formation in Composite Cryogels and the Influence of Electrical Stimulation.},
  url          = {http://dx.doi.org/10.7150/ijbs.13139},
  volume       = {11},
  year         = {2015},
}