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3 Genomic inventory of permafrost microorganisms

Mondav, Rhiannon LU orcid (2021) In Life in Extreme Environments 7. p.43-52
Abstract
The genomic inventory of a permafrost soil can be obtained through genomic characterization of the whole community (metagenomics) and/or an organism isolated from permafrost through culture or single-cell based methods. While there is often debate among the scientific community over whether genomic, transcriptomic, proteomic, or culture-based methods are best, the most promising current work combines knowledge from multiple culture-dependent and culture-independent approaches in comparative analyses of different permafrost and active-layer soils and nearby or related ecosystems. Because high-throughput shotgun methods of genomics and proteomics are relatively recent (and not always financially accessible to research groups), most... (More)
The genomic inventory of a permafrost soil can be obtained through genomic characterization of the whole community (metagenomics) and/or an organism isolated from permafrost through culture or single-cell based methods. While there is often debate among the scientific community over whether genomic, transcriptomic, proteomic, or culture-based methods are best, the most promising current work combines knowledge from multiple culture-dependent and culture-independent approaches in comparative analyses of different permafrost and active-layer soils and nearby or related ecosystems. Because high-throughput shotgun methods of genomics and proteomics are relatively recent (and not always financially accessible to research groups), most work done on permafrost communities has utilized phylogenetic surveys based on small sub-unit (SSU) rRNA and functional gene (e.g. mcrA, pmoA) surveys to characterize cryotic soil communities. In less than a decade, our knowledge of permafrost and cryosol communities has shifted from piece-meal to the beginnings of a holistic understanding. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
categories
Popular Science
host publication
Microbial Life in the Cryosphere and Its Feedback on Global Change
series title
Life in Extreme Environments
editor
Liebner, Susanne and Ganzert, Lars
volume
7
pages
43 - 52
publisher
De Gruyter
ISSN
2197-9227
2197-9235
ISBN
9783110497083
DOI
10.1515/9783110497083-003
language
English
LU publication?
no
id
82648951-045f-4676-8d4d-fd22ed1c54fb
date added to LUP
2023-06-05 16:11:25
date last changed
2023-06-10 03:09:54
@inbook{82648951-045f-4676-8d4d-fd22ed1c54fb,
  abstract     = {{The genomic inventory of a permafrost soil can be obtained through genomic characterization of the whole community (metagenomics) and/or an organism isolated from permafrost through culture or single-cell based methods. While there is often debate among the scientific community over whether genomic, transcriptomic, proteomic, or culture-based  methods  are  best,  the  most  promising  current  work  combines  knowledge from multiple culture-dependent and culture-independent approaches in comparative analyses of different permafrost and active-layer soils and nearby or related ecosystems. Because high-throughput shotgun methods of genomics and proteomics are relatively recent (and not always financially accessible to research groups), most work  done  on  permafrost  communities  has  utilized  phylogenetic  surveys  based  on  small sub-unit (SSU) rRNA and functional gene (e.g. mcrA, pmoA) surveys to characterize  cryotic  soil  communities.  In  less  than  a  decade,  our  knowledge  of  permafrost  and cryosol communities has shifted from piece-meal to the beginnings of a holistic understanding.}},
  author       = {{Mondav, Rhiannon}},
  booktitle    = {{Microbial Life in the Cryosphere and Its Feedback on Global Change}},
  editor       = {{Liebner, Susanne and Ganzert, Lars}},
  isbn         = {{9783110497083}},
  issn         = {{2197-9227}},
  language     = {{eng}},
  month        = {{01}},
  pages        = {{43--52}},
  publisher    = {{De Gruyter}},
  series       = {{Life in Extreme Environments}},
  title        = {{3 Genomic inventory of permafrost microorganisms}},
  url          = {{http://dx.doi.org/10.1515/9783110497083-003}},
  doi          = {{10.1515/9783110497083-003}},
  volume       = {{7}},
  year         = {{2021}},
}