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Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B

Robertsson, Carolina ; Svensäter, Gunnel ; Blum, Zoltan ; Jakobsson, Magnus E LU and Wickström, Claes LU (2021) In Journal of oral microbiology 13(1). p.1967636-1967636
Abstract

Background: Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic 'mucolytic' activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology.

Methods: The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and... (More)

Background: Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic 'mucolytic' activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology.

Methods: The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometry-based workflow, and differentially expressed proteins were identified.

Results and conclusions: Overall, the proteomic profiles of S. gordonii DL1 were similar across conditions. Six novel biofilm cell proteins and three planktonic proteins absent in all biofilm cultures were identified. These differences may provide insights into mechanisms for adaptation to biofilm growth in this species. Salivary MUC5B also elicited specific responses in the biofilm cell proteome. These regulations may represent mechanisms by which this mucin could promote colonization of the commensal S. gordonii in oral biofilms.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of oral microbiology
volume
13
issue
1
pages
1967636 - 1967636
publisher
Taylor & Francis
external identifiers
  • pmid:34447490
  • scopus:85120853173
ISSN
2000-2297
DOI
10.1080/20002297.2021.1967636
language
English
LU publication?
yes
additional info
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
id
8275ff9d-bc4d-4340-a997-0360bc944f83
date added to LUP
2021-11-07 19:59:15
date last changed
2024-06-15 20:02:02
@article{8275ff9d-bc4d-4340-a997-0360bc944f83,
  abstract     = {{<p>Background: Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic 'mucolytic' activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology.</p><p>Methods: The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometry-based workflow, and differentially expressed proteins were identified.</p><p>Results and conclusions: Overall, the proteomic profiles of S. gordonii DL1 were similar across conditions. Six novel biofilm cell proteins and three planktonic proteins absent in all biofilm cultures were identified. These differences may provide insights into mechanisms for adaptation to biofilm growth in this species. Salivary MUC5B also elicited specific responses in the biofilm cell proteome. These regulations may represent mechanisms by which this mucin could promote colonization of the commensal S. gordonii in oral biofilms.</p>}},
  author       = {{Robertsson, Carolina and Svensäter, Gunnel and Blum, Zoltan and Jakobsson, Magnus E and Wickström, Claes}},
  issn         = {{2000-2297}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{1967636--1967636}},
  publisher    = {{Taylor & Francis}},
  series       = {{Journal of oral microbiology}},
  title        = {{Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B}},
  url          = {{http://dx.doi.org/10.1080/20002297.2021.1967636}},
  doi          = {{10.1080/20002297.2021.1967636}},
  volume       = {{13}},
  year         = {{2021}},
}