High throughput continuous cell secretome separation inside microscale droplets by means of acoustophoresis
(2019) 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 p.902-903- Abstract
In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wide range of experimental parameters, an average separation efficiency of above 99% is achieved. The usability of the device for yeast cells is evaluated. This work demonstrates a missing key element for future high-throughput single-cell analysis techniques based on mass spectrometry.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/82b5de3c-28ef-49c4-96cb-1ad42d748b90
- author
- Gerlt, Michael LU ; Haidas, Dominik ; Ratschat, Alexandre ; Suter, Philipp ; Dittrich, Petra and Dual, Jürg
- publishing date
- 2019
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Cell manipulation, Cell secretome separation, Microfluidics, Microscale droplets
- host publication
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- series title
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
- conference location
- Basel, Switzerland
- conference dates
- 2019-10-27 - 2019-10-31
- external identifiers
-
- scopus:85094963201
- ISBN
- 9781733419000
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2019 CBMS-0001.
- id
- 82b5de3c-28ef-49c4-96cb-1ad42d748b90
- date added to LUP
- 2024-07-01 14:13:59
- date last changed
- 2024-07-11 14:52:20
@inproceedings{82b5de3c-28ef-49c4-96cb-1ad42d748b90, abstract = {{<p>In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wide range of experimental parameters, an average separation efficiency of above 99% is achieved. The usability of the device for yeast cells is evaluated. This work demonstrates a missing key element for future high-throughput single-cell analysis techniques based on mass spectrometry.</p>}}, author = {{Gerlt, Michael and Haidas, Dominik and Ratschat, Alexandre and Suter, Philipp and Dittrich, Petra and Dual, Jürg}}, booktitle = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}}, isbn = {{9781733419000}}, keywords = {{Cell manipulation; Cell secretome separation; Microfluidics; Microscale droplets}}, language = {{eng}}, pages = {{902--903}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}}, title = {{High throughput continuous cell secretome separation inside microscale droplets by means of acoustophoresis}}, year = {{2019}}, }