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High throughput continuous cell secretome separation inside microscale droplets by means of acoustophoresis

Gerlt, Michael LU orcid ; Haidas, Dominik ; Ratschat, Alexandre ; Suter, Philipp ; Dittrich, Petra and Dual, Jürg (2019) 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 p.902-903
Abstract

In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wide range of experimental parameters, an average separation efficiency of above 99% is achieved. The usability of the device for yeast cells is evaluated. This work demonstrates a missing key element for future high-throughput single-cell analysis techniques based on mass spectrometry.

Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Cell manipulation, Cell secretome separation, Microfluidics, Microscale droplets
host publication
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
series title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
conference location
Basel, Switzerland
conference dates
2019-10-27 - 2019-10-31
external identifiers
  • scopus:85094963201
ISBN
9781733419000
language
English
LU publication?
no
additional info
Publisher Copyright: © 2019 CBMS-0001.
id
82b5de3c-28ef-49c4-96cb-1ad42d748b90
date added to LUP
2024-07-01 14:13:59
date last changed
2024-07-11 14:52:20
@inproceedings{82b5de3c-28ef-49c4-96cb-1ad42d748b90,
  abstract     = {{<p>In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wide range of experimental parameters, an average separation efficiency of above 99% is achieved. The usability of the device for yeast cells is evaluated. This work demonstrates a missing key element for future high-throughput single-cell analysis techniques based on mass spectrometry.</p>}},
  author       = {{Gerlt, Michael and Haidas, Dominik and Ratschat, Alexandre and Suter, Philipp and Dittrich, Petra and Dual, Jürg}},
  booktitle    = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  isbn         = {{9781733419000}},
  keywords     = {{Cell manipulation; Cell secretome separation; Microfluidics; Microscale droplets}},
  language     = {{eng}},
  pages        = {{902--903}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019}},
  title        = {{High throughput continuous cell secretome separation inside microscale droplets by means of acoustophoresis}},
  year         = {{2019}},
}