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Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.

Broos, Sissela LU ; Lundberg, Kristina LU ; Akagi, Takami ; Kadowaki, Koji LU ; Akashi, Mitsuru ; Greiff, Lennart LU ; Borrebaeck, Carl LU and Lindstedt, Malin LU (2010) In Vaccine 28. p.5075-5085
Abstract
Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and... (More)
Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vaccine
volume
28
pages
5075 - 5085
publisher
Elsevier
external identifiers
  • pmid:20478343
  • wos:000280659600031
  • scopus:77954760985
  • pmid:20478343
ISSN
1873-2518
DOI
10.1016/j.vaccine.2010.05.004
language
English
LU publication?
yes
id
82b7bcc7-a99a-4f98-9ca9-2db85beefbbe (old id 1610149)
date added to LUP
2016-04-01 11:03:37
date last changed
2022-01-26 05:05:10
@article{82b7bcc7-a99a-4f98-9ca9-2db85beefbbe,
  abstract     = {{Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy.}},
  author       = {{Broos, Sissela and Lundberg, Kristina and Akagi, Takami and Kadowaki, Koji and Akashi, Mitsuru and Greiff, Lennart and Borrebaeck, Carl and Lindstedt, Malin}},
  issn         = {{1873-2518}},
  language     = {{eng}},
  pages        = {{5075--5085}},
  publisher    = {{Elsevier}},
  series       = {{Vaccine}},
  title        = {{Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.}},
  url          = {{http://dx.doi.org/10.1016/j.vaccine.2010.05.004}},
  doi          = {{10.1016/j.vaccine.2010.05.004}},
  volume       = {{28}},
  year         = {{2010}},
}