Identification and normalization of plate effects in cDNA Microarray data
(2002) In Preprints in Mathematical Sciences- Abstract
- Introducing a new way of visualizing cDNA microarray data we have identified a new type of systematic variation, which we refer to as plate effects. We believe that plate effects are due to non-biological differences in the cDNA clones products spotted onto the microarray slides. By comparing the consistency of all replicates (both within and between slides) after performing 42 different normalization strategies we claim that the plate effects arenon-negligible and should be corrected for in addition to the already known intensity dependent effects. For comparison between normalization strategies we introduce a novel robust genewise variability measure, which we call measure of reproducibility. In our case, we also found that not doing... (More)
- Introducing a new way of visualizing cDNA microarray data we have identified a new type of systematic variation, which we refer to as plate effects. We believe that plate effects are due to non-biological differences in the cDNA clones products spotted onto the microarray slides. By comparing the consistency of all replicates (both within and between slides) after performing 42 different normalization strategies we claim that the plate effects arenon-negligible and should be corrected for in addition to the already known intensity dependent effects. For comparison between normalization strategies we introduce a novel robust genewise variability measure, which we call measure of reproducibility. In our case, we also found that not doing background correction improves the reproducibility significantly. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/830048
- author
- Bengtsson, Henrik LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- unpublished
- subject
- in
- Preprints in Mathematical Sciences
- issue
- 2002:28
- publisher
- Lund University
- ISSN
- 1403-9338
- language
- English
- LU publication?
- yes
- id
- f7e8b3f5-5c6e-4501-ad95-343b82ad8f23 (old id 830048)
- date added to LUP
- 2016-04-04 09:26:21
- date last changed
- 2018-11-21 20:53:07
@article{f7e8b3f5-5c6e-4501-ad95-343b82ad8f23, abstract = {{Introducing a new way of visualizing cDNA microarray data we have identified a new type of systematic variation, which we refer to as plate effects. We believe that plate effects are due to non-biological differences in the cDNA clones products spotted onto the microarray slides. By comparing the consistency of all replicates (both within and between slides) after performing 42 different normalization strategies we claim that the plate effects arenon-negligible and should be corrected for in addition to the already known intensity dependent effects. For comparison between normalization strategies we introduce a novel robust genewise variability measure, which we call measure of reproducibility. In our case, we also found that not doing background correction improves the reproducibility significantly.}}, author = {{Bengtsson, Henrik}}, issn = {{1403-9338}}, language = {{eng}}, number = {{2002:28}}, publisher = {{Lund University}}, series = {{Preprints in Mathematical Sciences}}, title = {{Identification and normalization of plate effects in cDNA Microarray data}}, year = {{2002}}, }