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CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.

Scott, C L ; Bain, C C ; Wright, P B ; Sichien, D ; Kotarsky, Knut LU ; Persson, Emma LU ; Luda, Katarzyna LU ; Guilliams, M ; Lambrecht, B N and Agace, William LU , et al. (2015) In Mucosal Immunology 8(2). p.327-339
Abstract
The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the... (More)
The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mucosal Immunology
volume
8
issue
2
pages
327 - 339
publisher
Nature Publishing Group
external identifiers
  • pmid:25138666
  • wos:000349681200010
  • scopus:84922714497
  • pmid:25138666
ISSN
1933-0219
DOI
10.1038/mi.2014.70
language
English
LU publication?
yes
id
8312672c-817a-4aa8-b216-017b1dfdcfae (old id 4614296)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25138666?dopt=Abstract
date added to LUP
2016-04-01 10:06:10
date last changed
2022-02-17 06:35:52
@article{8312672c-817a-4aa8-b216-017b1dfdcfae,
  abstract     = {{The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70.}},
  author       = {{Scott, C L and Bain, C C and Wright, P B and Sichien, D and Kotarsky, Knut and Persson, Emma and Luda, Katarzyna and Guilliams, M and Lambrecht, B N and Agace, William and Milling, S Wf and Mowat, Allan}},
  issn         = {{1933-0219}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{327--339}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Mucosal Immunology}},
  title        = {{CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.}},
  url          = {{http://dx.doi.org/10.1038/mi.2014.70}},
  doi          = {{10.1038/mi.2014.70}},
  volume       = {{8}},
  year         = {{2015}},
}