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Integrated Brain Atlas for Unbiased Mapping of Nervous System Effects Following Liraglutide Treatment

Salinas, Casper Bo Gravesen ; Lu, Tess Tsai-Hsiu ; Gabery, Sanaz LU ; Marstal, Kasper ; Alanentalo, Tomas ; Mercer, Aaron Jeffrey ; Cornea, Anda ; Conradsen, Knut ; Hecksher-Sørensen, Jacob and Dahl, Anders Bjorholm , et al. (2018) In Scientific Reports 8(1).
Abstract

Light Sheet Fluorescence Microscopy (LSFM) of whole organs, in particular the brain, offers a plethora of biological data imaged in 3D. This technique is however often hindered by cumbersome non-automated analysis methods. Here we describe an approach to fully automate the analysis by integrating with data from the Allen Institute of Brain Science (AIBS), to provide precise assessment of the distribution and action of peptide-based pharmaceuticals in the brain. To illustrate this approach, we examined the acute central nervous system effects of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Peripherally administered liraglutide accessed the hypothalamus and brainstem, and led to activation in several brain regions of... (More)

Light Sheet Fluorescence Microscopy (LSFM) of whole organs, in particular the brain, offers a plethora of biological data imaged in 3D. This technique is however often hindered by cumbersome non-automated analysis methods. Here we describe an approach to fully automate the analysis by integrating with data from the Allen Institute of Brain Science (AIBS), to provide precise assessment of the distribution and action of peptide-based pharmaceuticals in the brain. To illustrate this approach, we examined the acute central nervous system effects of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Peripherally administered liraglutide accessed the hypothalamus and brainstem, and led to activation in several brain regions of which most were intersected by projections from neurons in the lateral parabrachial nucleus. Collectively, we provide a rapid and unbiased analytical framework for LSFM data which enables quantification and exploration based on data from AIBS to support basic and translational discovery.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
article number
10310
pages
12 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:29985439
  • scopus:85049856872
ISSN
2045-2322
DOI
10.1038/s41598-018-28496-6
language
English
LU publication?
yes
id
832ec516-246e-4334-9596-db22e61a2af9
date added to LUP
2019-02-04 13:44:55
date last changed
2021-04-20 02:56:44
@article{832ec516-246e-4334-9596-db22e61a2af9,
  abstract     = {<p>Light Sheet Fluorescence Microscopy (LSFM) of whole organs, in particular the brain, offers a plethora of biological data imaged in 3D. This technique is however often hindered by cumbersome non-automated analysis methods. Here we describe an approach to fully automate the analysis by integrating with data from the Allen Institute of Brain Science (AIBS), to provide precise assessment of the distribution and action of peptide-based pharmaceuticals in the brain. To illustrate this approach, we examined the acute central nervous system effects of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Peripherally administered liraglutide accessed the hypothalamus and brainstem, and led to activation in several brain regions of which most were intersected by projections from neurons in the lateral parabrachial nucleus. Collectively, we provide a rapid and unbiased analytical framework for LSFM data which enables quantification and exploration based on data from AIBS to support basic and translational discovery.</p>},
  author       = {Salinas, Casper Bo Gravesen and Lu, Tess Tsai-Hsiu and Gabery, Sanaz and Marstal, Kasper and Alanentalo, Tomas and Mercer, Aaron Jeffrey and Cornea, Anda and Conradsen, Knut and Hecksher-Sørensen, Jacob and Dahl, Anders Bjorholm and Knudsen, Lotte Bjerre and Secher, Anna},
  issn         = {2045-2322},
  language     = {eng},
  month        = {07},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Integrated Brain Atlas for Unbiased Mapping of Nervous System Effects Following Liraglutide Treatment},
  url          = {http://dx.doi.org/10.1038/s41598-018-28496-6},
  doi          = {10.1038/s41598-018-28496-6},
  volume       = {8},
  year         = {2018},
}