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Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards

Segovia, Karen M.; Stallknecht, David E.; Kapczynski, Darrell R.; Stabler, Lisa; Berghaus, Roy D.; Fotjik, Alinde; Latorre-Margalef, Neus LU and França, Monique S. (2017) In PLoS ONE 12(1).
Abstract

Mallards are widely recognized as reservoirs for Influenza A viruses (IAV); however, host factors that might prompt seasonality and trends in subtype diversity of IAV such as adaptive heterosubtypic immunity (HSI) are not well understood. To investigate this, we inoculated mallards with a prevailing H3N8 low pathogenic avian influenza virus (LPAIV) subtype in waterfowl to determine if prior infection with this virus would be protective against heterosubtypic infections with the H4N6, H10N7 and H14N5 LPAIV subtypes after one, two and three months, respectively. Also, we investigated the effect of cumulative immunity after sequential inoculation of mallards with these viruses in one-month intervals. Humoral immunity was assessed by... (More)

Mallards are widely recognized as reservoirs for Influenza A viruses (IAV); however, host factors that might prompt seasonality and trends in subtype diversity of IAV such as adaptive heterosubtypic immunity (HSI) are not well understood. To investigate this, we inoculated mallards with a prevailing H3N8 low pathogenic avian influenza virus (LPAIV) subtype in waterfowl to determine if prior infection with this virus would be protective against heterosubtypic infections with the H4N6, H10N7 and H14N5 LPAIV subtypes after one, two and three months, respectively. Also, we investigated the effect of cumulative immunity after sequential inoculation of mallards with these viruses in one-month intervals. Humoral immunity was assessed by microneutralization assays using a subset of representative LPAIV subtypes as antigens. Our results indicate that prior inoculation with the H3N8 virus confers partial protective immunity against subsequent heterosubtypic infections with the robustness of HSI related to the phylogenetic similarity of the HA protein of the strains used. Furthermore, induced HSI was boosted and followed by repeated exposure to more than one LPAIV subtype. Our findings provide further information on the contributions of HSI and its role in the dynamics of IAV subtype diversity in mallards.

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author
publishing date
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publication status
published
subject
in
PLoS ONE
volume
12
issue
1
publisher
Public Library of Science
external identifiers
  • scopus:85010451182
ISSN
1932-6203
DOI
10.1371/journal.pone.0170335
language
English
LU publication?
no
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839b226a-fee7-4e3d-bf4d-fa3491e283ff
date added to LUP
2017-04-11 13:53:13
date last changed
2018-05-06 04:32:25
@article{839b226a-fee7-4e3d-bf4d-fa3491e283ff,
  abstract     = {<p>Mallards are widely recognized as reservoirs for Influenza A viruses (IAV); however, host factors that might prompt seasonality and trends in subtype diversity of IAV such as adaptive heterosubtypic immunity (HSI) are not well understood. To investigate this, we inoculated mallards with a prevailing H3N8 low pathogenic avian influenza virus (LPAIV) subtype in waterfowl to determine if prior infection with this virus would be protective against heterosubtypic infections with the H4N6, H10N7 and H14N5 LPAIV subtypes after one, two and three months, respectively. Also, we investigated the effect of cumulative immunity after sequential inoculation of mallards with these viruses in one-month intervals. Humoral immunity was assessed by microneutralization assays using a subset of representative LPAIV subtypes as antigens. Our results indicate that prior inoculation with the H3N8 virus confers partial protective immunity against subsequent heterosubtypic infections with the robustness of HSI related to the phylogenetic similarity of the HA protein of the strains used. Furthermore, induced HSI was boosted and followed by repeated exposure to more than one LPAIV subtype. Our findings provide further information on the contributions of HSI and its role in the dynamics of IAV subtype diversity in mallards.</p>},
  articleno    = {e0170335},
  author       = {Segovia, Karen M. and Stallknecht, David E. and Kapczynski, Darrell R. and Stabler, Lisa and Berghaus, Roy D. and Fotjik, Alinde and Latorre-Margalef, Neus and França, Monique S.},
  issn         = {1932-6203},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards},
  url          = {http://dx.doi.org/10.1371/journal.pone.0170335},
  volume       = {12},
  year         = {2017},
}