Structural characterization of the stringent response related exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family
(2004) In Biochemistry 43(28). p.8894-8900- Abstract
- Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GPPA family. The protein has a two-domain structure with an active site located in the interdomain cleft. Two crystal forms were investigated (type I and 11) at resolutions of 1.53 and 2.15 Angstrom, respectively. This revealed a structural flexibility that has previously been described as a "butterfly-like" cleft opening around the active site in... (More)
- Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GPPA family. The protein has a two-domain structure with an active site located in the interdomain cleft. Two crystal forms were investigated (type I and 11) at resolutions of 1.53 and 2.15 Angstrom, respectively. This revealed a structural flexibility that has previously been described as a "butterfly-like" cleft opening around the active site in other actin-like superfamily proteins. A calcium ion is observed at the center of this region in type I crystals, substantiating that PPX/GPPA enzymes use metal ions for catalysis. Structural analysis suggests that nucleotides bind at a similar position to that seen in other members of the superfamily. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/139386
- author
- Kristensen, O ; Laurberg, Martin LU ; Liljas, Anders LU ; Kastrup, J S and Gajhede, M
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemistry
- volume
- 43
- issue
- 28
- pages
- 8894 - 8900
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000222668000002
- pmid:15248747
- scopus:3142666890
- ISSN
- 0006-2960
- DOI
- 10.1021/bi049083c
- language
- English
- LU publication?
- yes
- id
- 83aeb631-fb9e-4b20-8945-26cea5e3b5d2 (old id 139386)
- date added to LUP
- 2016-04-01 11:36:16
- date last changed
- 2022-01-26 07:31:52
@article{83aeb631-fb9e-4b20-8945-26cea5e3b5d2, abstract = {{Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GPPA family. The protein has a two-domain structure with an active site located in the interdomain cleft. Two crystal forms were investigated (type I and 11) at resolutions of 1.53 and 2.15 Angstrom, respectively. This revealed a structural flexibility that has previously been described as a "butterfly-like" cleft opening around the active site in other actin-like superfamily proteins. A calcium ion is observed at the center of this region in type I crystals, substantiating that PPX/GPPA enzymes use metal ions for catalysis. Structural analysis suggests that nucleotides bind at a similar position to that seen in other members of the superfamily.}}, author = {{Kristensen, O and Laurberg, Martin and Liljas, Anders and Kastrup, J S and Gajhede, M}}, issn = {{0006-2960}}, language = {{eng}}, number = {{28}}, pages = {{8894--8900}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biochemistry}}, title = {{Structural characterization of the stringent response related exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family}}, url = {{http://dx.doi.org/10.1021/bi049083c}}, doi = {{10.1021/bi049083c}}, volume = {{43}}, year = {{2004}}, }