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Expression of Integrin A2 Receptor in Human Cord Blood Cd34+Cd38-Cd90+ Stem Cells Engrafting Long-Term in Nod/Scid-Il2rγ(C) Null Mice.

Wong, Wan Man LU ; Sigvardsson, Mikael LU ; Åstrand-Grundström, Ingbritt LU ; Hogge, Donna ; Larsson, Jonas LU ; Qian, Hong LU and Ekblom, Marja LU (2013) In Stem Cells 31(2). p.360-371
Abstract
Human hematopoietic stem cells reside in the CD34+CD38-CD90+ population in cord blood and bone marrow. However, this cell fraction is heterogeneous, and the phenotype of the rare primitive stem cells remains poorly defined. We here report that primitive cord blood CD34+CD38-CD90+ stem cells, with the ability to reconstitute NOD/SCID-IL2Rγ(c) null mice long term, at 24 weeks after transplantation, can be prospectively isolated at an increased purity by using integrin α2 receptor as an additional stem cell marker. Using a limiting dilution transplantation assay, we found a highly significant enrichment of multilineage reconstituting stem cells in the CD34+CD38-CD90+ cell fraction expressing the integrin α2 receptor, with a frequency of 1/29... (More)
Human hematopoietic stem cells reside in the CD34+CD38-CD90+ population in cord blood and bone marrow. However, this cell fraction is heterogeneous, and the phenotype of the rare primitive stem cells remains poorly defined. We here report that primitive cord blood CD34+CD38-CD90+ stem cells, with the ability to reconstitute NOD/SCID-IL2Rγ(c) null mice long term, at 24 weeks after transplantation, can be prospectively isolated at an increased purity by using integrin α2 receptor as an additional stem cell marker. Using a limiting dilution transplantation assay, we found a highly significant enrichment of multilineage reconstituting stem cells in the CD34+CD38-CD90+ cell fraction expressing the integrin α2 receptor, with a frequency of 1/29 cells, as compared to a frequency of 1/157 in the corresponding integrin α2- cells. In line with this, long-term reconstituting stem cells within the cord blood CD34+CD38- cell population were significantly enriched in the integrin α2+ fraction, while stem cells and progenitors reconstituting short-term, at 8-12 weeks, were heterogeneous in integrin α2 expression. Global gene expression profiling revealed that the lineage-marker negative (Lin-) CD34+CD38-CD90+CD45RA- integrin α2+ cell population was molecularly distinct from the integrin α2- cell population and the more mature Lin-CD34+CD38-CD90-CD45RA- cell population. Our findings identify integrin α2 as a novel stem cell marker, which improves prospective isolation of the primitive human hematopoietic stem cells within the CD34+CD38-CD90+ cell population for experimental and therapeutic stem cell applications. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fetal blood, Hematopoietic stem cells, Integrin alpha2, Human
in
Stem Cells
volume
31
issue
2
pages
360 - 371
publisher
AlphaMed Press
external identifiers
  • wos:000314873000015
  • pmid:23165626
  • scopus:84874343988
  • pmid:23165626
ISSN
1549-4918
DOI
10.1002/stem.1282
language
English
LU publication?
yes
id
83b33597-1135-441c-b4cd-5eef283fa234 (old id 3218790)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23165626?dopt=Abstract
date added to LUP
2016-04-01 13:28:10
date last changed
2020-01-12 12:24:36
@article{83b33597-1135-441c-b4cd-5eef283fa234,
  abstract     = {Human hematopoietic stem cells reside in the CD34+CD38-CD90+ population in cord blood and bone marrow. However, this cell fraction is heterogeneous, and the phenotype of the rare primitive stem cells remains poorly defined. We here report that primitive cord blood CD34+CD38-CD90+ stem cells, with the ability to reconstitute NOD/SCID-IL2Rγ(c) null mice long term, at 24 weeks after transplantation, can be prospectively isolated at an increased purity by using integrin α2 receptor as an additional stem cell marker. Using a limiting dilution transplantation assay, we found a highly significant enrichment of multilineage reconstituting stem cells in the CD34+CD38-CD90+ cell fraction expressing the integrin α2 receptor, with a frequency of 1/29 cells, as compared to a frequency of 1/157 in the corresponding integrin α2- cells. In line with this, long-term reconstituting stem cells within the cord blood CD34+CD38- cell population were significantly enriched in the integrin α2+ fraction, while stem cells and progenitors reconstituting short-term, at 8-12 weeks, were heterogeneous in integrin α2 expression. Global gene expression profiling revealed that the lineage-marker negative (Lin-) CD34+CD38-CD90+CD45RA- integrin α2+ cell population was molecularly distinct from the integrin α2- cell population and the more mature Lin-CD34+CD38-CD90-CD45RA- cell population. Our findings identify integrin α2 as a novel stem cell marker, which improves prospective isolation of the primitive human hematopoietic stem cells within the CD34+CD38-CD90+ cell population for experimental and therapeutic stem cell applications.},
  author       = {Wong, Wan Man and Sigvardsson, Mikael and Åstrand-Grundström, Ingbritt and Hogge, Donna and Larsson, Jonas and Qian, Hong and Ekblom, Marja},
  issn         = {1549-4918},
  language     = {eng},
  number       = {2},
  pages        = {360--371},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Expression of Integrin A2 Receptor in Human Cord Blood Cd34+Cd38-Cd90+ Stem Cells Engrafting Long-Term in Nod/Scid-Il2rγ(C) Null Mice.},
  url          = {http://dx.doi.org/10.1002/stem.1282},
  doi          = {10.1002/stem.1282},
  volume       = {31},
  year         = {2013},
}