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Construction of a Chassis for a Tripartite Protein-Based Molecular Motor

Small, Lara S.R.; Bruning, Marc; Thomson, Andrew R.; Boyle, Aimee L.; Davies, Roberta B.; Curmi, Paul M G; Forde, Nancy R.; Linke, Heiner LU ; Woolfson, Derek N and Bromley, Elizabeth H. C. (2017) In ACS Synthetic Biology 6(6). p.1096-1102
Abstract

Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering, and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the structure designed for the Tumbleweed... (More)

Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering, and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the structure designed for the Tumbleweed motor. The successful formation of a suitable hub structure is both a test of the transferability of design rules for protein folding as well as an important step in the production of a synthetic protein-based molecular motor.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Synthetic Biology
volume
6
issue
6
pages
7 pages
publisher
American Chemical Society (ACS)
external identifiers
  • scopus:85020892104
  • wos:000403864900019
ISSN
2161-5063
DOI
10.1021/acssynbio.7b00037
language
English
LU publication?
yes
id
83db0976-983d-45df-9993-8da024db8617
date added to LUP
2017-07-04 08:27:55
date last changed
2018-04-04 03:00:31
@article{83db0976-983d-45df-9993-8da024db8617,
  abstract     = {<p>Improving our understanding of biological motors, both to fully comprehend their activities in vital processes, and to exploit their impressive abilities for use in bionanotechnology, is highly desirable. One means of understanding these systems is through the production of synthetic molecular motors. We demonstrate the use of orthogonal coiled-coil dimers (including both parallel and antiparallel coiled coils) as a hub for linking other components of a previously described synthetic molecular motor, the Tumbleweed. We use circular dichroism, analytical ultracentrifugation, dynamic light scattering, and disulfide rearrangement studies to demonstrate the ability of this six-peptide set to form the structure designed for the Tumbleweed motor. The successful formation of a suitable hub structure is both a test of the transferability of design rules for protein folding as well as an important step in the production of a synthetic protein-based molecular motor.</p>},
  author       = {Small, Lara S.R. and Bruning, Marc and Thomson, Andrew R. and Boyle, Aimee L. and Davies, Roberta B. and Curmi, Paul M G and Forde, Nancy R. and Linke, Heiner and Woolfson, Derek N and Bromley, Elizabeth H. C.},
  issn         = {2161-5063},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {1096--1102},
  publisher    = {American Chemical Society (ACS)},
  series       = {ACS Synthetic Biology},
  title        = {Construction of a Chassis for a Tripartite Protein-Based Molecular Motor},
  url          = {http://dx.doi.org/10.1021/acssynbio.7b00037},
  volume       = {6},
  year         = {2017},
}