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Retinal glial cell immunoreactivity and neuronal cell changes in rats with STZ-induced diabetes

Agardh, Elisabet LU ; Bruun, Anitha LU and Agardh, Carl-David LU (2001) In Current Eye Research 23(4). p.276-284
Abstract
PURPOSE: To study whether diabetes could influence glial cells, retinal neurons, and pigment epithelial cells and if so, to evaluate whether any changes could be influenced by aminoguanidine (AG) or probucol (PB). METHODS: Streptozocin (STZ)-induced diabetic male Wistar rats and age-matched control rats were fed a normal diet, addition of AG in the drinking water (0.5 g/l for diabetic and 1.0 g/l for control rats) or PB in the pellets (1 % w/w) for one or six months. Paraffin embedded retinal sections were incubated in the primary antibodies GFAP, calbindin, RPE65, and Hu, for glial, horizontal, pigment epithelial, and ganglion cells, respectively, and in fluorescent secondary antibodies. RESULTS: One month after STZ injection, GFAP... (More)
PURPOSE: To study whether diabetes could influence glial cells, retinal neurons, and pigment epithelial cells and if so, to evaluate whether any changes could be influenced by aminoguanidine (AG) or probucol (PB). METHODS: Streptozocin (STZ)-induced diabetic male Wistar rats and age-matched control rats were fed a normal diet, addition of AG in the drinking water (0.5 g/l for diabetic and 1.0 g/l for control rats) or PB in the pellets (1 % w/w) for one or six months. Paraffin embedded retinal sections were incubated in the primary antibodies GFAP, calbindin, RPE65, and Hu, for glial, horizontal, pigment epithelial, and ganglion cells, respectively, and in fluorescent secondary antibodies. RESULTS: One month after STZ injection, GFAP immunoreactivity was sparse, but after six months it was prominent in glial cells in 5/5 diabetic and 1/7 control retinas (p = 0.015). Neither AG, nor PB influenced this immunoreactivity. Numbers of retinal pigment epithelial cells and cells in the ganglion cell layer, were similar at one and six months of diabetes. By time, the number of horizontal cells decreased (p < 0.001) and branching and numbers of their terminals were reduced (p < 0.001). CONCLUSION: Diabetes for six months resulted in increased glial cell immunoreactivity, and by age, horizontal cell numbers and branching of their terminals decreased, morphological patterns that were unaffected by AG or PB. The numbers of retinal pigment epithelial cells and cells in the ganglion cell layer were unaffected both by age and diabetes. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Eye Research
volume
23
issue
4
pages
276 - 284
publisher
Taylor & Francis
external identifiers
  • scopus:0036175576
  • pmid:11852429
ISSN
0271-3683
DOI
10.1076/ceyr.23.4.276.5459
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Unit on Vascular Diabetic Complications (013241510), Ophthalmology (Lund) (013043000)
id
840c9a7b-7cf7-41cd-8b12-6ca3a919f188 (old id 1124865)
date added to LUP
2016-04-01 11:53:09
date last changed
2025-04-04 14:32:51
@article{840c9a7b-7cf7-41cd-8b12-6ca3a919f188,
  abstract     = {{PURPOSE: To study whether diabetes could influence glial cells, retinal neurons, and pigment epithelial cells and if so, to evaluate whether any changes could be influenced by aminoguanidine (AG) or probucol (PB). METHODS: Streptozocin (STZ)-induced diabetic male Wistar rats and age-matched control rats were fed a normal diet, addition of AG in the drinking water (0.5 g/l for diabetic and 1.0 g/l for control rats) or PB in the pellets (1 % w/w) for one or six months. Paraffin embedded retinal sections were incubated in the primary antibodies GFAP, calbindin, RPE65, and Hu, for glial, horizontal, pigment epithelial, and ganglion cells, respectively, and in fluorescent secondary antibodies. RESULTS: One month after STZ injection, GFAP immunoreactivity was sparse, but after six months it was prominent in glial cells in 5/5 diabetic and 1/7 control retinas (p = 0.015). Neither AG, nor PB influenced this immunoreactivity. Numbers of retinal pigment epithelial cells and cells in the ganglion cell layer, were similar at one and six months of diabetes. By time, the number of horizontal cells decreased (p &lt; 0.001) and branching and numbers of their terminals were reduced (p &lt; 0.001). CONCLUSION: Diabetes for six months resulted in increased glial cell immunoreactivity, and by age, horizontal cell numbers and branching of their terminals decreased, morphological patterns that were unaffected by AG or PB. The numbers of retinal pigment epithelial cells and cells in the ganglion cell layer were unaffected both by age and diabetes.}},
  author       = {{Agardh, Elisabet and Bruun, Anitha and Agardh, Carl-David}},
  issn         = {{0271-3683}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{276--284}},
  publisher    = {{Taylor & Francis}},
  series       = {{Current Eye Research}},
  title        = {{Retinal glial cell immunoreactivity and neuronal cell changes in rats with STZ-induced diabetes}},
  url          = {{http://dx.doi.org/10.1076/ceyr.23.4.276.5459}},
  doi          = {{10.1076/ceyr.23.4.276.5459}},
  volume       = {{23}},
  year         = {{2001}},
}