Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release
Kromann, H. ; Christy, M. ; Egeberg, J. ; Lernmark, A. LU
; Nerup, J.
LU
and Richter-Olesen, H.
(1980)
In Medical Biology
58(6).
p.322-328
- Abstract
Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of... (More)
Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.
(Less)
- author
- Kromann, H.
; Christy, M.
; Egeberg, J.
; Lernmark, A.
LU
; Nerup, J.
LU
and Richter-Olesen, H.
- publishing date
- 1980-12-01
- type
- Contribution to journal
- publication status
- published
- in
- Medical Biology
- volume
- 58
- issue
- 6
- pages
- 322 - 328
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:6453261
- scopus:0019271975
- ISSN
- 0302-2137
- language
- English
- LU publication?
- no
- id
- 843f6b5c-4883-48b0-8a66-1511dc9fa636
- date added to LUP
- 2019-09-16 15:37:06
- date last changed
- 2024-03-13 07:59:49
@article{843f6b5c-4883-48b0-8a66-1511dc9fa636,
abstract = {{<p>Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 <sup>5</sup> cells with 10 <sup>6</sup> cpm of [ <sup>51</sup>]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ <sup>51</sup>]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ <sup>51</sup>]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.</p>}},
author = {{Kromann, H. and Christy, M. and Egeberg, J. and Lernmark, A. and Nerup, J. and Richter-Olesen, H.}},
issn = {{0302-2137}},
language = {{eng}},
month = {{12}},
number = {{6}},
pages = {{322--328}},
publisher = {{Taylor & Francis}},
series = {{Medical Biology}},
title = {{Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ <sup>51</sup>]CR-release}},
volume = {{58}},
year = {{1980}},
}