[The age-related characteristics of the hormonal regulation of Na+, K(+)-ATPase activity in the kidney cortex of rats]
(1992) In Biulleten' eksperimental'noi biologii i meditsiny 114(8). p.3-150- Abstract
The aldosterone binding in isolated distal convoluted and cortical collecting tubules of renal nephrons and the influence of hormonal induction on the Na, K-ATPase activity in membrane fraction of kidney cortex were studied in 10-day- and 2-month-old rats. No reliable difference in aldosterone-specific binding was revealed (0.26 +/- 0.04 and 0.22 +/- 0.03 fmol/mm of tubule length, respectively, at the age of 10 days and 2 months). It was found that Na, K-ATPase activity increased with age from 0.39 +/- 0.06 to 0.72 +/- 0.10 mumol Pi/mg of protein.1 hour.100 microliters. Aldosterone induction caused approximately a 3-fold increase of the enzyme activity in both age groups comparing to the control level. Co-induction of aldosterone and... (More)
The aldosterone binding in isolated distal convoluted and cortical collecting tubules of renal nephrons and the influence of hormonal induction on the Na, K-ATPase activity in membrane fraction of kidney cortex were studied in 10-day- and 2-month-old rats. No reliable difference in aldosterone-specific binding was revealed (0.26 +/- 0.04 and 0.22 +/- 0.03 fmol/mm of tubule length, respectively, at the age of 10 days and 2 months). It was found that Na, K-ATPase activity increased with age from 0.39 +/- 0.06 to 0.72 +/- 0.10 mumol Pi/mg of protein.1 hour.100 microliters. Aldosterone induction caused approximately a 3-fold increase of the enzyme activity in both age groups comparing to the control level. Co-induction of aldosterone and spironolactone resulted in a 50% decrease of Na, K-ATPase activity in adult rats, but did not influence that in young rats. The revealed age-related differences in the mechanism of hormonal Na, K-ATPase regulation are supposed to underlie the absence of physiological reaction of the kidney to aldosterone in early postnatal ontogenesis.
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- author
- Svitasheva, N G LU ; Kalish, E E ; Logvinenko, N S and Solenov, E I
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adrenalectomy, Aging/drug effects, Aldosterone/metabolism, Animals, Kidney Cortex/drug effects, Kidney Tubules, Collecting/drug effects, Kidney Tubules, Distal/drug effects, Male, Mifepristone/pharmacology, Rats, Rats, Wistar, Receptors, Mineralocorticoid/drug effects, Sodium-Potassium-Exchanging ATPase/drug effects, Spironolactone/pharmacology
- in
- Biulleten' eksperimental'noi biologii i meditsiny
- volume
- 114
- issue
- 8
- pages
- 3 - 150
- publisher
- Rossiiskaya Akademiya Meditsinskikh Nauk
- external identifiers
-
- scopus:0026540674
- pmid:1334727
- ISSN
- 0365-9615
- language
- English
- LU publication?
- no
- id
- 848c7d2a-b9b8-452e-9096-d72e677342db
- date added to LUP
- 2020-03-02 09:10:34
- date last changed
- 2024-01-02 06:59:15
@article{848c7d2a-b9b8-452e-9096-d72e677342db,
abstract = {{<p>The aldosterone binding in isolated distal convoluted and cortical collecting tubules of renal nephrons and the influence of hormonal induction on the Na, K-ATPase activity in membrane fraction of kidney cortex were studied in 10-day- and 2-month-old rats. No reliable difference in aldosterone-specific binding was revealed (0.26 +/- 0.04 and 0.22 +/- 0.03 fmol/mm of tubule length, respectively, at the age of 10 days and 2 months). It was found that Na, K-ATPase activity increased with age from 0.39 +/- 0.06 to 0.72 +/- 0.10 mumol Pi/mg of protein.1 hour.100 microliters. Aldosterone induction caused approximately a 3-fold increase of the enzyme activity in both age groups comparing to the control level. Co-induction of aldosterone and spironolactone resulted in a 50% decrease of Na, K-ATPase activity in adult rats, but did not influence that in young rats. The revealed age-related differences in the mechanism of hormonal Na, K-ATPase regulation are supposed to underlie the absence of physiological reaction of the kidney to aldosterone in early postnatal ontogenesis.</p>}},
author = {{Svitasheva, N G and Kalish, E E and Logvinenko, N S and Solenov, E I}},
issn = {{0365-9615}},
keywords = {{Adrenalectomy; Aging/drug effects; Aldosterone/metabolism; Animals; Kidney Cortex/drug effects; Kidney Tubules, Collecting/drug effects; Kidney Tubules, Distal/drug effects; Male; Mifepristone/pharmacology; Rats; Rats, Wistar; Receptors, Mineralocorticoid/drug effects; Sodium-Potassium-Exchanging ATPase/drug effects; Spironolactone/pharmacology}},
language = {{eng}},
number = {{8}},
pages = {{3--150}},
publisher = {{Rossiiskaya Akademiya Meditsinskikh Nauk}},
series = {{Biulleten' eksperimental'noi biologii i meditsiny}},
title = {{[The age-related characteristics of the hormonal regulation of Na+, K(+)-ATPase activity in the kidney cortex of rats]}},
volume = {{114}},
year = {{1992}},
}