Advanced

Exploring the prebiotic effect of cyclodextrins on probiotic bacteria entrapped in carboxymetyl cellulose-chitosan particles

Lindman, Björn LU ; Medronho, Bruno LU ; Miguel, Maria LU ; Singh, Poonam and Valente, Artur (2018) In Colloids and Surfaces. B, Biointerfaces 168. p.156-162
Abstract
n this work the prebiotic effect of different cyclodextrins, CDs, on the viability of model probiotic bacteria (Lactobacillus rhamnosus GG) encapsulated in carboxymethyl cellulose-chitosan (CMC-Cht) hybrid particles was studied. All the CDs tested were observed to considerably improve the viability (quantitatively like common prebiotics, such as corn starch) and encapsulation efficiency when compared to the CD-free particles, as inferred by plate counting method and fluorescence microscopy. The SEM data suggests that the morphology of the particles, the roughness of the surface and porosity, are dependent on the type of CD and may reflect different interactions between the CDs and the matrix components. The aging and stability of the... (More)
n this work the prebiotic effect of different cyclodextrins, CDs, on the viability of model probiotic bacteria (Lactobacillus rhamnosus GG) encapsulated in carboxymethyl cellulose-chitosan (CMC-Cht) hybrid particles was studied. All the CDs tested were observed to considerably improve the viability (quantitatively like common prebiotics, such as corn starch) and encapsulation efficiency when compared to the CD-free particles, as inferred by plate counting method and fluorescence microscopy. The SEM data suggests that the morphology of the particles, the roughness of the surface and porosity, are dependent on the type of CD and may reflect different interactions between the CDs and the matrix components. The aging and stability of the samples with and without beta-CD were further evaluated. Remarkably, the viability count of the CD-doped samples was still reasonably high after one month storage at room temperature with acceptable values for practical uses. Moreover, when the CMC-Cht particles were exposed to in vitro simulated digestion fluids, the cell survival was much enhanced when the particles contained beta-CD. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces. B, Biointerfaces
volume
168
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85039897467
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2017.12.014
language
English
LU publication?
no
id
849f70ab-e5d1-417a-b2cc-81d88b0a0678
date added to LUP
2019-01-03 19:40:41
date last changed
2019-05-21 04:16:58
@article{849f70ab-e5d1-417a-b2cc-81d88b0a0678,
  abstract     = {n this work the prebiotic effect of different cyclodextrins, CDs, on the viability of model probiotic bacteria (Lactobacillus rhamnosus GG) encapsulated in carboxymethyl cellulose-chitosan (CMC-Cht) hybrid particles was studied. All the CDs tested were observed to considerably improve the viability (quantitatively like common prebiotics, such as corn starch) and encapsulation efficiency when compared to the CD-free particles, as inferred by plate counting method and fluorescence microscopy. The SEM data suggests that the morphology of the particles, the roughness of the surface and porosity, are dependent on the type of CD and may reflect different interactions between the CDs and the matrix components. The aging and stability of the samples with and without beta-CD were further evaluated. Remarkably, the viability count of the CD-doped samples was still reasonably high after one month storage at room temperature with acceptable values for practical uses. Moreover, when the CMC-Cht particles were exposed to in vitro simulated digestion fluids, the cell survival was much enhanced when the particles contained beta-CD. },
  author       = {Lindman, Björn and Medronho, Bruno and Miguel, Maria and Singh, Poonam and Valente, Artur},
  issn         = {1873-4367},
  language     = {eng},
  pages        = {156--162},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces. B, Biointerfaces},
  title        = {Exploring the prebiotic effect of cyclodextrins on probiotic bacteria entrapped in carboxymetyl cellulose-chitosan particles},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2017.12.014},
  volume       = {168},
  year         = {2018},
}