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Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation

Marin Navarro, Ana ; Day, Kelly ; Kogner, Per ; Wilhelm, Margareta and Falk, Anna LU (2019) In Stem Cell Research 34.
Abstract

Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anaplastic Lymphoma Kinase/genetics, Base Sequence, Cell Culture Techniques/methods, Cell Line, Germ-Line Mutation/genetics, Humans, Induced Pluripotent Stem Cells/pathology, Male, Neuroblastoma/genetics
in
Stem Cell Research
volume
34
article number
101356
pages
4 pages
publisher
Elsevier
external identifiers
  • pmid:30605844
  • scopus:85059227913
ISSN
1876-7753
DOI
10.1016/j.scr.2018.11.017
language
English
LU publication?
no
id
84c19201-e172-47b4-903d-e1bd13654d1d
date added to LUP
2021-08-09 15:56:10
date last changed
2024-05-18 12:50:52
@article{84c19201-e172-47b4-903d-e1bd13654d1d,
  abstract     = {{<p>Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers.</p>}},
  author       = {{Marin Navarro, Ana and Day, Kelly and Kogner, Per and Wilhelm, Margareta and Falk, Anna}},
  issn         = {{1876-7753}},
  keywords     = {{Anaplastic Lymphoma Kinase/genetics; Base Sequence; Cell Culture Techniques/methods; Cell Line; Germ-Line Mutation/genetics; Humans; Induced Pluripotent Stem Cells/pathology; Male; Neuroblastoma/genetics}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Stem Cell Research}},
  title        = {{Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation}},
  url          = {{https://lup.lub.lu.se/search/files/101036154/Generation_of_induced_pluripotent_stem_cell.pdf}},
  doi          = {{10.1016/j.scr.2018.11.017}},
  volume       = {{34}},
  year         = {{2019}},
}