Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation
(2019) In Stem Cell Research 34.- Abstract
Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/84c19201-e172-47b4-903d-e1bd13654d1d
- author
- Marin Navarro, Ana ; Day, Kelly ; Kogner, Per ; Wilhelm, Margareta and Falk, Anna LU
- publishing date
- 2019-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Anaplastic Lymphoma Kinase/genetics, Base Sequence, Cell Culture Techniques/methods, Cell Line, Germ-Line Mutation/genetics, Humans, Induced Pluripotent Stem Cells/pathology, Male, Neuroblastoma/genetics
- in
- Stem Cell Research
- volume
- 34
- article number
- 101356
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:30605844
- scopus:85059227913
- ISSN
- 1876-7753
- DOI
- 10.1016/j.scr.2018.11.017
- language
- English
- LU publication?
- no
- id
- 84c19201-e172-47b4-903d-e1bd13654d1d
- date added to LUP
- 2021-08-09 15:56:10
- date last changed
- 2024-08-24 21:15:03
@article{84c19201-e172-47b4-903d-e1bd13654d1d, abstract = {{<p>Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers.</p>}}, author = {{Marin Navarro, Ana and Day, Kelly and Kogner, Per and Wilhelm, Margareta and Falk, Anna}}, issn = {{1876-7753}}, keywords = {{Anaplastic Lymphoma Kinase/genetics; Base Sequence; Cell Culture Techniques/methods; Cell Line; Germ-Line Mutation/genetics; Humans; Induced Pluripotent Stem Cells/pathology; Male; Neuroblastoma/genetics}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation}}, url = {{https://lup.lub.lu.se/search/files/101036154/Generation_of_induced_pluripotent_stem_cell.pdf}}, doi = {{10.1016/j.scr.2018.11.017}}, volume = {{34}}, year = {{2019}}, }