Analysis of the immunochemical relationship between antigens by electrophoresis in agarose gels containing antibodies
Grubb, A LU
(1972)
In Scandinavian journal of clinical and laboratory investigation. Supplementum
124.
p.59-65
- Abstract
- A procedure is described, in which two antigens are brought to interact during their electrophoretic migration in an agarose gel containing antiserum. Three fundamental precipitation patterns can be discerned. These patterns correspond to the three basic precipitation patterns obtained with the double radial immunodiffusion procedure of Ouchterlony. The procedure described here can thus be used in the analysis of the immunochemical relationsship between antigens. It may also be used for estimating the percentage of cross-reacting antibodies in an antiserum. The procedure requires only a few hours.
Please use this url to cite or link to this publication:
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- author
- Grubb, A
LU
- organization
- publishing date
- 1972
- type
- Contribution to journal
- publication status
- published
- keywords
- Agar, Animals, Antibodies, Antigens/analysis, Chemical Precipitation, Cross Reactions, Dogs, Electrophoresis, Disc, Haplorhini, Haptoglobins/analysis, Humans, Immunodiffusion
- in
- Scandinavian journal of clinical and laboratory investigation. Supplementum
- volume
- 124
- pages
- 59 - 65
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0015270117
- pmid:4625210
- ISSN
- 0085-591X
- DOI
- 10.3109/00365517209102752
- language
- English
- LU publication?
- yes
- id
- 84c5faaa-db99-482a-ad4e-92ff29ca4379
- date added to LUP
- 2021-10-16 19:05:58
- date last changed
- 2024-01-12 02:54:46
@article{84c5faaa-db99-482a-ad4e-92ff29ca4379,
abstract = {{A procedure is described, in which two antigens are brought to interact during their electrophoretic migration in an agarose gel containing antiserum. Three fundamental precipitation patterns can be discerned. These patterns correspond to the three basic precipitation patterns obtained with the double radial immunodiffusion procedure of Ouchterlony. The procedure described here can thus be used in the analysis of the immunochemical relationsship between antigens. It may also be used for estimating the percentage of cross-reacting antibodies in an antiserum. The procedure requires only a few hours.}},
author = {{Grubb, A}},
issn = {{0085-591X}},
keywords = {{Agar; Animals; Antibodies; Antigens/analysis; Chemical Precipitation; Cross Reactions; Dogs; Electrophoresis, Disc; Haplorhini; Haptoglobins/analysis; Humans; Immunodiffusion}},
language = {{eng}},
pages = {{59--65}},
publisher = {{Taylor & Francis}},
series = {{Scandinavian journal of clinical and laboratory investigation. Supplementum}},
title = {{Analysis of the immunochemical relationship between antigens by electrophoresis in agarose gels containing antibodies}},
url = {{http://dx.doi.org/10.3109/00365517209102752}},
doi = {{10.3109/00365517209102752}},
volume = {{124}},
year = {{1972}},
}