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Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa

Abrahim, Saro Abdella ; Girma, Mulu ; Habteselassie, Abebe ; Gezahegn, Nigussie ; Feleke, Altaye ; Berheto, Tezera Moshago ; Demissie, Minilik LU ; Belete, Wudnesh and Deressa, Tekalign (2019) In HIV/AIDS (Auckland, N.Z.) 11. p.17-22
Abstract

Purpose: Genetic diversities in different countries affect the performance of HIV test kits. Therefore, WHO recommends evaluation of every HIV test kit in countries' context before its use. Therefore, this study aimed to evaluate the performance of Genscreen ULTRA HIV Ag-Ab and Bioelisa.

Materials and methods: The study had used 400 characterized plasma samples obtained from CDC Atlanta bio-bank derived from Africa, USA, and Thailand.

Results: Diagnostic performance of both test kits under evaluation was assessed at 95% CI. Genscreen ULTRA HIV Ag-Ab had sensitivity and negative predictive value of 99.5% [95% CI, 97.2-99.9] and the specificity and positive predictive value of 98.5% [95% CI, 95.7-99.7]. Bioelisa HIV test kit... (More)

Purpose: Genetic diversities in different countries affect the performance of HIV test kits. Therefore, WHO recommends evaluation of every HIV test kit in countries' context before its use. Therefore, this study aimed to evaluate the performance of Genscreen ULTRA HIV Ag-Ab and Bioelisa.

Materials and methods: The study had used 400 characterized plasma samples obtained from CDC Atlanta bio-bank derived from Africa, USA, and Thailand.

Results: Diagnostic performance of both test kits under evaluation was assessed at 95% CI. Genscreen ULTRA HIV Ag-Ab had sensitivity and negative predictive value of 99.5% [95% CI, 97.2-99.9] and the specificity and positive predictive value of 98.5% [95% CI, 95.7-99.7]. Bioelisa HIV test kit had exhibited sensitivity and negative predictive value of 99% [95% CI, 96.4-99.7] and specificity and positive predictive value of 98.5% [95% CI, 95.7-99.7]. Both test kits were able to detect almost all samples with HIV-2, dual infections, and seroconversion.

Conclusion: Both the test kits were highly sensitive and specific in detecting HIV. However, there are still few samples containing HIV antibody which were not identified by both kits. Therefore, additional screening measures should be done in using these assays for blood transfusion and organ transplantation. In addition, the study can be used as a reference by other African countries.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
HIV/AIDS (Auckland, N.Z.)
volume
11
pages
17 - 22
publisher
Dove Medical Press Ltd.
external identifiers
  • scopus:85077901098
  • pmid:30804685
ISSN
1179-1373
DOI
10.2147/HIV.S184603
language
English
LU publication?
no
id
84e0a4f8-5f16-49ea-b608-2b49e2b103c9
date added to LUP
2019-09-12 09:56:32
date last changed
2021-02-17 05:57:21
@article{84e0a4f8-5f16-49ea-b608-2b49e2b103c9,
  abstract     = {<p>Purpose: Genetic diversities in different countries affect the performance of HIV test kits. Therefore, WHO recommends evaluation of every HIV test kit in countries' context before its use. Therefore, this study aimed to evaluate the performance of Genscreen ULTRA HIV Ag-Ab and Bioelisa.</p><p>Materials and methods: The study had used 400 characterized plasma samples obtained from CDC Atlanta bio-bank derived from Africa, USA, and Thailand.</p><p>Results: Diagnostic performance of both test kits under evaluation was assessed at 95% CI. Genscreen ULTRA HIV Ag-Ab had sensitivity and negative predictive value of 99.5% [95% CI, 97.2-99.9] and the specificity and positive predictive value of 98.5% [95% CI, 95.7-99.7]. Bioelisa HIV test kit had exhibited sensitivity and negative predictive value of 99% [95% CI, 96.4-99.7] and specificity and positive predictive value of 98.5% [95% CI, 95.7-99.7]. Both test kits were able to detect almost all samples with HIV-2, dual infections, and seroconversion.</p><p>Conclusion: Both the test kits were highly sensitive and specific in detecting HIV. However, there are still few samples containing HIV antibody which were not identified by both kits. Therefore, additional screening measures should be done in using these assays for blood transfusion and organ transplantation. In addition, the study can be used as a reference by other African countries.</p>},
  author       = {Abrahim, Saro Abdella and Girma, Mulu and Habteselassie, Abebe and Gezahegn, Nigussie and Feleke, Altaye and Berheto, Tezera Moshago and Demissie, Minilik and Belete, Wudnesh and Deressa, Tekalign},
  issn         = {1179-1373},
  language     = {eng},
  pages        = {17--22},
  publisher    = {Dove Medical Press Ltd.},
  series       = {HIV/AIDS (Auckland, N.Z.)},
  title        = {Diagnostic accuracy of HIV test kits, Genscreen Ultra and Bioelisa},
  url          = {http://dx.doi.org/10.2147/HIV.S184603},
  doi          = {10.2147/HIV.S184603},
  volume       = {11},
  year         = {2019},
}