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Fast, automated, continuous energy scans for experimental phasing at the BioMAX beamline

Gorgisyan, Ishkhan LU ; Bell, Paul LU ; Cascella, Michele LU orcid ; Eguiraun, Mikel LU ; Freitas, Áureo LU ; Lidon-Simon, Julio LU ; Nan, Jie LU ; Takahashi, Carla LU ; Tarawneh, Hamed LU and Ursby, Thomas LU , et al. (2023) In Journal of Synchrotron Radiation 30. p.885-894
Abstract

In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously... (More)

In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously operational at BioMAX. The implementation of the continuous energy scan facilitates the prompt access to the anomalous scattering data, required for the SAD and MAD experiments.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
continuous energy scan, flying scan, MAD SAD phasing, motion synchronization, MX beamline
in
Journal of Synchrotron Radiation
volume
30
pages
10 pages
publisher
International Union of Crystallography
external identifiers
  • scopus:85169848290
  • pmid:37526994
ISSN
0909-0495
DOI
10.1107/S1600577523005738
language
English
LU publication?
yes
id
84f5e1ca-4237-4a65-8da8-f4c7320429fc
date added to LUP
2023-10-24 14:26:49
date last changed
2024-06-28 09:15:15
@article{84f5e1ca-4237-4a65-8da8-f4c7320429fc,
  abstract     = {{<p>In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously operational at BioMAX. The implementation of the continuous energy scan facilitates the prompt access to the anomalous scattering data, required for the SAD and MAD experiments.</p>}},
  author       = {{Gorgisyan, Ishkhan and Bell, Paul and Cascella, Michele and Eguiraun, Mikel and Freitas, Áureo and Lidon-Simon, Julio and Nan, Jie and Takahashi, Carla and Tarawneh, Hamed and Ursby, Thomas and Gonzalez, Ana}},
  issn         = {{0909-0495}},
  keywords     = {{continuous energy scan; flying scan; MAD SAD phasing; motion synchronization; MX beamline}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{885--894}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{Fast, automated, continuous energy scans for experimental phasing at the BioMAX beamline}},
  url          = {{http://dx.doi.org/10.1107/S1600577523005738}},
  doi          = {{10.1107/S1600577523005738}},
  volume       = {{30}},
  year         = {{2023}},
}