Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.
(2016) In Methods in Molecular Biology 1377. p.413-420- Abstract
- Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8503909
- author
- Sitsel, Oleg ; Wang, Kaituo ; Liu, Xiangyu and Gourdon, Pontus LU
- organization
- publishing date
- 2016
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- P-Type ATPases
- series title
- Methods in Molecular Biology
- editor
- Bublitz, Maike
- volume
- 1377
- pages
- 413 - 420
- external identifiers
-
- pmid:26695052
- scopus:84951019140
- pmid:26695052
- ISSN
- 1064-3745
- ISBN
- 978-1-4939-3178-1
- DOI
- 10.1007/978-1-4939-3179-8_37
- language
- English
- LU publication?
- yes
- id
- 88b04c5d-085b-43e9-a966-1ab75a2e993f (old id 8503909)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26695052?dopt=Abstract
- date added to LUP
- 2016-04-04 08:06:40
- date last changed
- 2022-04-24 07:55:38
@inbook{88b04c5d-085b-43e9-a966-1ab75a2e993f, abstract = {{Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.}}, author = {{Sitsel, Oleg and Wang, Kaituo and Liu, Xiangyu and Gourdon, Pontus}}, booktitle = {{P-Type ATPases}}, editor = {{Bublitz, Maike}}, isbn = {{978-1-4939-3178-1}}, issn = {{1064-3745}}, language = {{eng}}, pages = {{413--420}}, series = {{Methods in Molecular Biology}}, title = {{Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.}}, url = {{http://dx.doi.org/10.1007/978-1-4939-3179-8_37}}, doi = {{10.1007/978-1-4939-3179-8_37}}, volume = {{1377}}, year = {{2016}}, }