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Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.

Sitsel, Oleg ; Wang, Kaituo ; Liu, Xiangyu and Gourdon, Pontus LU (2016) In Methods in Molecular Biology 1377. p.413-420
Abstract
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
P-Type ATPases
series title
Methods in Molecular Biology
editor
Bublitz, Maike
volume
1377
pages
413 - 420
external identifiers
  • pmid:26695052
  • scopus:84951019140
  • pmid:26695052
ISSN
1064-3745
ISBN
978-1-4939-3178-1
DOI
10.1007/978-1-4939-3179-8_37
language
English
LU publication?
yes
id
88b04c5d-085b-43e9-a966-1ab75a2e993f (old id 8503909)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26695052?dopt=Abstract
date added to LUP
2016-04-04 08:06:40
date last changed
2022-04-24 07:55:38
@inbook{88b04c5d-085b-43e9-a966-1ab75a2e993f,
  abstract     = {{Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.}},
  author       = {{Sitsel, Oleg and Wang, Kaituo and Liu, Xiangyu and Gourdon, Pontus}},
  booktitle    = {{P-Type ATPases}},
  editor       = {{Bublitz, Maike}},
  isbn         = {{978-1-4939-3178-1}},
  issn         = {{1064-3745}},
  language     = {{eng}},
  pages        = {{413--420}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-3179-8_37}},
  doi          = {{10.1007/978-1-4939-3179-8_37}},
  volume       = {{1377}},
  year         = {{2016}},
}