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Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.

Sitsel, Oleg; Wang, Kaituo; Liu, Xiangyu and Gourdon, Pontus LU (2016) In P-Type ATPases 1377. p.413-420
Abstract
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
P-Type ATPases
editor
Bublitz, Maike and
volume
1377
pages
413 - 420
external identifiers
  • pmid:26695052
  • scopus:84951019140
ISSN
1064-3745
ISBN
978-1-4939-3178-1
DOI
10.1007/978-1-4939-3179-8_37
language
English
LU publication?
yes
id
88b04c5d-085b-43e9-a966-1ab75a2e993f (old id 8503909)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26695052?dopt=Abstract
date added to LUP
2016-01-12 12:39:33
date last changed
2017-04-29 15:27:47
@inbook{88b04c5d-085b-43e9-a966-1ab75a2e993f,
  abstract     = {Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.},
  author       = {Sitsel, Oleg and Wang, Kaituo and Liu, Xiangyu and Gourdon, Pontus},
  editor       = {Bublitz, Maike},
  isbn         = {978-1-4939-3178-1},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {413--420},
  series       = {P-Type ATPases},
  title        = {Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3179-8_37},
  volume       = {1377},
  year         = {2016},
}