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Overproduction of PIB-Type ATPases.

Liu, Xiangyu; Sitsel, Oleg; Wang, Kaituo and Gourdon, Pontus LU (2016) In Methods in Molecular Biology 1377. p.29-36
Abstract
Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1377
pages
29 - 36
publisher
Springer
external identifiers
  • pmid:26695020
  • scopus:84951081850
ISSN
1940-6029
DOI
10.1007/978-1-4939-3179-8_5
language
English
LU publication?
yes
id
05065485-317b-4905-bcdb-f51e1d678e93 (old id 8503916)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26695020?dopt=Abstract
date added to LUP
2016-01-12 12:38:38
date last changed
2017-04-29 15:26:50
@article{05065485-317b-4905-bcdb-f51e1d678e93,
  abstract     = {Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.},
  author       = {Liu, Xiangyu and Sitsel, Oleg and Wang, Kaituo and Gourdon, Pontus},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {29--36},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Overproduction of PIB-Type ATPases.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3179-8_5},
  volume       = {1377},
  year         = {2016},
}