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From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.

Persson, Henrik; Li, Zhen; Tegenfeldt, Jonas O; Oredsson, Stina and Prinz, Christelle LU (2015) In Scientific Reports 5.
Abstract
The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the... (More)
The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing. (Less)
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author
organization
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Contribution to journal
publication status
published
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in
Scientific Reports
volume
5
publisher
Nature Publishing Group
external identifiers
  • pmid:26691936
  • wos:000367039100001
  • scopus:84951731872
ISSN
2045-2322
DOI
10.1038/srep18535
language
English
LU publication?
yes
id
02e17ba9-2e47-4f34-9e6e-f7cd6c1d59f4 (old id 8504016)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26691936?dopt=Abstract
date added to LUP
2016-01-06 12:12:48
date last changed
2017-10-01 04:09:31
@article{02e17ba9-2e47-4f34-9e6e-f7cd6c1d59f4,
  abstract     = {The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing.},
  articleno    = {18535},
  author       = {Persson, Henrik and Li, Zhen and Tegenfeldt, Jonas O and Oredsson, Stina and Prinz, Christelle},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.},
  url          = {http://dx.doi.org/10.1038/srep18535},
  volume       = {5},
  year         = {2015},
}