From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.
Persson, Henrik ; Li, Zhen LU ; Tegenfeldt, Jonas O LU
; Oredsson, Stina
LU
and Prinz, Christelle
LU
(2015)
In Scientific Reports
5.
- Abstract
- The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the... (More)
- The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8504016
- author
- Persson, Henrik
; Li, Zhen
LU
; Tegenfeldt, Jonas O
LU
; Oredsson, Stina
LU
and Prinz, Christelle
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 5
- article number
- 18535
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:26691936
- wos:000367039100001
- scopus:84951731872
- pmid:26691936
- ISSN
- 2045-2322
- DOI
- 10.1038/srep18535
- language
- English
- LU publication?
- yes
- id
- 02e17ba9-2e47-4f34-9e6e-f7cd6c1d59f4 (old id 8504016)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26691936?dopt=Abstract
- date added to LUP
- 2016-04-01 13:33:54
- date last changed
- 2023-11-12 18:35:10
@article{02e17ba9-2e47-4f34-9e6e-f7cd6c1d59f4,
abstract = {{The field of vertical nanowire array-based applications in cell biology is growing rapidly and an increasing number of applications are being explored. These applications almost invariably rely on the physical properties of the nanowire arrays, creating a need for a better understanding of how their physical properties affect cell behaviour. Here, we investigate the effects of nanowire density on cell migration, division and morphology for murine fibroblasts. Our results show that few nanowires are sufficient to immobilize cells, while a high nanowire spatial density enables a "bed-of-nails" regime, where cells reside on top of the nanowires and are fully motile. The presence of nanowires decreases the cell proliferation rate, even in the "bed-of-nails" regime. We show that the cell morphology strongly depends on the nanowire density. Cells cultured on low (0.1 μm(-2)) and medium (1 μm(-2)) density substrates exhibit an increased number of multi-nucleated cells and micronuclei. These were not observed in cells cultured on high nanowire density substrates (4 μm(-2)). The results offer important guidelines to minimize cell-function perturbations on nanowire arrays. Moreover, these findings offer the possibility to tune cell proliferation and migration independently by adjusting the nanowire density, which may have applications in drug testing.}},
author = {{Persson, Henrik and Li, Zhen and Tegenfeldt, Jonas O and Oredsson, Stina and Prinz, Christelle}},
issn = {{2045-2322}},
language = {{eng}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.}},
url = {{http://dx.doi.org/10.1038/srep18535}},
doi = {{10.1038/srep18535}},
volume = {{5}},
year = {{2015}},
}