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Strong maternal effects on gene expression in Arabidopsis lyrata hybrids.

Videvall, Elin LU ; Sletvold, Nina; Hagenblad, Jenny; Ågren, Jon and Hansson, Bengt LU (2015) In Molecular Biology and Evolution 33(4). p.984-994
Abstract
Hybridization between populations or species can have pronounced fitness consequences. Yet little is known about how hybridization affects gene regulation. Three main models have been put forward to explain gene expression patterns in hybrids: additive, dominance or parental effects. Here we use high throughput RNA-sequencing to examine the extent to which hybrid gene expression follows predictions by each of the three models. We performed a reciprocal crossing experiment between two differentiated populations of the perennial herb Arabidopsis lyrata and sequenced RNA in rosette leaves of twelve-week-old plants grown in greenhouse conditions. The two parental populations had highly differentiated gene expression patterns. In hybrids, a... (More)
Hybridization between populations or species can have pronounced fitness consequences. Yet little is known about how hybridization affects gene regulation. Three main models have been put forward to explain gene expression patterns in hybrids: additive, dominance or parental effects. Here we use high throughput RNA-sequencing to examine the extent to which hybrid gene expression follows predictions by each of the three models. We performed a reciprocal crossing experiment between two differentiated populations of the perennial herb Arabidopsis lyrata and sequenced RNA in rosette leaves of twelve-week-old plants grown in greenhouse conditions. The two parental populations had highly differentiated gene expression patterns. In hybrids, a majority of genes showed intermediate expression relative to that of their parental populations (i.e. additive effects), but expression was frequently more similar to the maternal than to their paternal population (i.e. maternal effects). Allele-specific expression analyses showed that in the vast majority of cases, genes with pronounced maternal effect expressed both the maternal and the paternal allele. Maternal effects on hybrid gene expression have rarely been documented previously and our study suggests it could be more common than previously assumed. Whether the maternal effect on gene expression persists to later life-stages, and whether the variation in gene expression is manifested in other aspects of the phenotype, remain to be elucidated. Our findings are relevant for understanding the consequences of outbreeding and hybridization and open up several questions for future studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Biology and Evolution
volume
33
issue
4
pages
984 - 994
publisher
Oxford University Press
external identifiers
  • pmid:26685177
  • wos:000374226700011
  • scopus:84964858732
ISSN
0737-4038
DOI
10.1093/molbev/msv342
language
English
LU publication?
yes
id
348b092b-ade7-4fda-be4a-bb7404d8c962 (old id 8504166)
date added to LUP
2016-01-08 09:26:56
date last changed
2017-11-19 03:14:59
@article{348b092b-ade7-4fda-be4a-bb7404d8c962,
  abstract     = {Hybridization between populations or species can have pronounced fitness consequences. Yet little is known about how hybridization affects gene regulation. Three main models have been put forward to explain gene expression patterns in hybrids: additive, dominance or parental effects. Here we use high throughput RNA-sequencing to examine the extent to which hybrid gene expression follows predictions by each of the three models. We performed a reciprocal crossing experiment between two differentiated populations of the perennial herb Arabidopsis lyrata and sequenced RNA in rosette leaves of twelve-week-old plants grown in greenhouse conditions. The two parental populations had highly differentiated gene expression patterns. In hybrids, a majority of genes showed intermediate expression relative to that of their parental populations (i.e. additive effects), but expression was frequently more similar to the maternal than to their paternal population (i.e. maternal effects). Allele-specific expression analyses showed that in the vast majority of cases, genes with pronounced maternal effect expressed both the maternal and the paternal allele. Maternal effects on hybrid gene expression have rarely been documented previously and our study suggests it could be more common than previously assumed. Whether the maternal effect on gene expression persists to later life-stages, and whether the variation in gene expression is manifested in other aspects of the phenotype, remain to be elucidated. Our findings are relevant for understanding the consequences of outbreeding and hybridization and open up several questions for future studies.},
  author       = {Videvall, Elin and Sletvold, Nina and Hagenblad, Jenny and Ågren, Jon and Hansson, Bengt},
  issn         = {0737-4038},
  language     = {eng},
  month        = {12},
  number       = {4},
  pages        = {984--994},
  publisher    = {Oxford University Press},
  series       = {Molecular Biology and Evolution},
  title        = {Strong maternal effects on gene expression in Arabidopsis lyrata hybrids.},
  url          = {http://dx.doi.org/10.1093/molbev/msv342},
  volume       = {33},
  year         = {2015},
}