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Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.

Rödström, Karin LU and Lindkvist, Karin LU (2016) In Methods in Molecular Biology 1396. p.51-65
Abstract
Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1396
pages
51 - 65
publisher
Springer
external identifiers
  • pmid:26676036
  • scopus:84950279250
ISSN
1940-6029
DOI
10.1007/978-1-4939-3344-0_4
language
English
LU publication?
yes
id
1037d161-ce36-41ce-b905-06396a6fe4c3 (old id 8504444)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26676036?dopt=Abstract
date added to LUP
2016-01-12 12:28:56
date last changed
2017-02-14 16:15:59
@article{1037d161-ce36-41ce-b905-06396a6fe4c3,
  abstract     = {Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.},
  author       = {Rödström, Karin and Lindkvist, Karin},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {51--65},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3344-0_4},
  volume       = {1396},
  year         = {2016},
}