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Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits.

Mandal, Nakul LU ; Lewis, Geoffrey P; Fisher, Steven K; Heegaard, Steffen; Prause, Jan U; la Cour, Morten; Vorum, Henrik and Honoré, Bent (2015) In Journal of Ophthalmology 2015.
Abstract
Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis... (More)
Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry. Results. Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups. Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications. (Less)
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Contribution to journal
publication status
published
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in
Journal of Ophthalmology
volume
2015
publisher
Hindawi Publishing Corporation
external identifiers
  • pmid:26664739
  • wos:000365548100001
  • scopus:84948808647
ISSN
2090-0058
DOI
10.1155/2015/583040
language
English
LU publication?
yes
id
a8be8bf9-0511-4279-afd3-d52f13637a83 (old id 8504861)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26664739?dopt=Abstract
date added to LUP
2016-01-05 19:14:49
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2017-11-05 03:07:16
@article{a8be8bf9-0511-4279-afd3-d52f13637a83,
  abstract     = {Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry. Results. Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups. Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications.},
  articleno    = {583040},
  author       = {Mandal, Nakul and Lewis, Geoffrey P and Fisher, Steven K and Heegaard, Steffen and Prause, Jan U and la Cour, Morten and Vorum, Henrik and Honoré, Bent},
  issn         = {2090-0058},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of Ophthalmology},
  title        = {Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits.},
  url          = {http://dx.doi.org/10.1155/2015/583040},
  volume       = {2015},
  year         = {2015},
}