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Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids.

Lind, Tania LU ; Wacklin, Hanna LU ; Schiller, Jürgen; Moulin, Martine; Haertlein, Michael; Pomorski, Thomas Günther and Cárdenas, Marité (2015) In PLoS ONE 10(12).
Abstract
Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported lipid bilayers from total lipid extracts of Escherichia coli by vesicle fusion. We show the validity of this method for different types of extracts including those from deuterated biomass using a combination of complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection and atomic force microscopy. We find that the head group composition of the deuterated and the hydrogenated lipid extracts is similar (approximately 75% phosphatidylethanolamine, 13% phosphatidylglycerol and 12% cardiolipin) and that both samples can be used to... (More)
Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported lipid bilayers from total lipid extracts of Escherichia coli by vesicle fusion. We show the validity of this method for different types of extracts including those from deuterated biomass using a combination of complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection and atomic force microscopy. We find that the head group composition of the deuterated and the hydrogenated lipid extracts is similar (approximately 75% phosphatidylethanolamine, 13% phosphatidylglycerol and 12% cardiolipin) and that both samples can be used to reconstitute high-coverage supported lipid bilayers with a total thickness of 41 ± 3 Å, common for fluid membranes. The formation of supported lipid bilayers composed of natural extracts of Escherichia coli allow for following biomolecular interactions, thus advancing the field towards bacterial-specific membrane biomimics. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
10
issue
12
publisher
Public Library of Science
external identifiers
  • pmid:26658241
  • wos:000366903600075
  • scopus:84957108270
ISSN
1932-6203
DOI
10.1371/journal.pone.0144671
language
English
LU publication?
yes
id
7e19f47e-a717-4a2b-b2dc-317aed9ebd67 (old id 8505136)
date added to LUP
2016-01-08 09:18:52
date last changed
2017-11-12 03:38:02
@article{7e19f47e-a717-4a2b-b2dc-317aed9ebd67,
  abstract     = {Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported lipid bilayers from total lipid extracts of Escherichia coli by vesicle fusion. We show the validity of this method for different types of extracts including those from deuterated biomass using a combination of complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection and atomic force microscopy. We find that the head group composition of the deuterated and the hydrogenated lipid extracts is similar (approximately 75% phosphatidylethanolamine, 13% phosphatidylglycerol and 12% cardiolipin) and that both samples can be used to reconstitute high-coverage supported lipid bilayers with a total thickness of 41 ± 3 Å, common for fluid membranes. The formation of supported lipid bilayers composed of natural extracts of Escherichia coli allow for following biomolecular interactions, thus advancing the field towards bacterial-specific membrane biomimics.},
  articleno    = {e0144671},
  author       = {Lind, Tania and Wacklin, Hanna and Schiller, Jürgen and Moulin, Martine and Haertlein, Michael and Pomorski, Thomas Günther and Cárdenas, Marité},
  issn         = {1932-6203},
  language     = {eng},
  number       = {12},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0144671},
  volume       = {10},
  year         = {2015},
}