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Transplant experiments uncover Baltic Sea basin-specific responses in bacterioplankton community composition and metabolic activities

Lindh, Markus V LU ; Figueroa, Daniela ; Sjöstedt, Johanna LU ; Baltar, Federico ; Lundin, Daniel ; Andersson, Agneta ; Legrand, Catherine and Pinhassi, Jarone (2015) In Frontiers in Microbiology 6.
Abstract

Anthropogenically induced changes in precipitation are projected to generate increased river runoff to semi-enclosed seas, increasing loads of terrestrial dissolved organic matter and decreasing salinity. To determine how bacterial community structure and functioning adjust to such changes, we designed microcosm transplant experiments with Baltic Proper (salinity 7.2) and Bothnian Sea (salinity 3.6) water. Baltic Proper bacteria generally reached higher abundances than Bothnian Sea bacteria in both Baltic Proper and Bothnian Sea water, indicating higher adaptability. Moreover, Baltic Proper bacteria growing in Bothnian Sea water consistently showed highest bacterial production and beta-glucosidase activity. These metabolic responses... (More)

Anthropogenically induced changes in precipitation are projected to generate increased river runoff to semi-enclosed seas, increasing loads of terrestrial dissolved organic matter and decreasing salinity. To determine how bacterial community structure and functioning adjust to such changes, we designed microcosm transplant experiments with Baltic Proper (salinity 7.2) and Bothnian Sea (salinity 3.6) water. Baltic Proper bacteria generally reached higher abundances than Bothnian Sea bacteria in both Baltic Proper and Bothnian Sea water, indicating higher adaptability. Moreover, Baltic Proper bacteria growing in Bothnian Sea water consistently showed highest bacterial production and beta-glucosidase activity. These metabolic responses were accompanied by basin-specific changes in bacterial community structure. For example, Baltic Proper Pseudomonas and Limnobacter populations increased markedly in relative abundance in Bothnian Sea water, indicating a replacement effect. In contrast, Roseobacter and Rheinheimera populations were stable or increased in abundance when challenged by either of the waters, indicating an adjustment effect. Transplants to Bothnian Sea water triggered the initial emergence of particular Burkholderiaceae populations, and transplants to Baltic Proper water triggered Alteromonadaceae populations. Notably, in the subsequent re-transplant experiment, a priming effect resulted in further increases to dominance of these populations. Correlated changes in community composition and metabolic activity were observed only in the transplant experiment and only at relatively high phylogenetic resolution. This suggested an importance of successional progression for interpreting relationships between bacterial community composition and functioning. We infer that priming effects on bacterial community structure by natural episodic events or climate change induced forcing could translate into long-term changes in bacterial ecosystem process rates.

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author
publishing date
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Contribution to journal
publication status
published
in
Frontiers in Microbiology
volume
6
article number
223
pages
18 pages
publisher
Frontiers
external identifiers
  • scopus:84930943030
  • pmid:25883589
ISSN
1664-302X
DOI
10.3389/fmicb.2015.00223
language
English
LU publication?
no
id
8506b18c-0953-47fe-b057-d16bd8662bf8
date added to LUP
2019-05-15 14:43:47
date last changed
2019-12-10 08:04:25
@article{8506b18c-0953-47fe-b057-d16bd8662bf8,
  abstract     = {<p>Anthropogenically induced changes in precipitation are projected to generate increased river runoff to semi-enclosed seas, increasing loads of terrestrial dissolved organic matter and decreasing salinity. To determine how bacterial community structure and functioning adjust to such changes, we designed microcosm transplant experiments with Baltic Proper (salinity 7.2) and Bothnian Sea (salinity 3.6) water. Baltic Proper bacteria generally reached higher abundances than Bothnian Sea bacteria in both Baltic Proper and Bothnian Sea water, indicating higher adaptability. Moreover, Baltic Proper bacteria growing in Bothnian Sea water consistently showed highest bacterial production and beta-glucosidase activity. These metabolic responses were accompanied by basin-specific changes in bacterial community structure. For example, Baltic Proper Pseudomonas and Limnobacter populations increased markedly in relative abundance in Bothnian Sea water, indicating a replacement effect. In contrast, Roseobacter and Rheinheimera populations were stable or increased in abundance when challenged by either of the waters, indicating an adjustment effect. Transplants to Bothnian Sea water triggered the initial emergence of particular Burkholderiaceae populations, and transplants to Baltic Proper water triggered Alteromonadaceae populations. Notably, in the subsequent re-transplant experiment, a priming effect resulted in further increases to dominance of these populations. Correlated changes in community composition and metabolic activity were observed only in the transplant experiment and only at relatively high phylogenetic resolution. This suggested an importance of successional progression for interpreting relationships between bacterial community composition and functioning. We infer that priming effects on bacterial community structure by natural episodic events or climate change induced forcing could translate into long-term changes in bacterial ecosystem process rates. </p>},
  author       = {Lindh, Markus V and Figueroa, Daniela and Sjöstedt, Johanna and Baltar, Federico and Lundin, Daniel and Andersson, Agneta and Legrand, Catherine and Pinhassi, Jarone},
  issn         = {1664-302X},
  language     = {eng},
  month        = {04},
  publisher    = {Frontiers},
  series       = {Frontiers in Microbiology},
  title        = {Transplant experiments uncover Baltic Sea basin-specific responses in bacterioplankton community composition and metabolic activities},
  url          = {http://dx.doi.org/10.3389/fmicb.2015.00223},
  doi          = {10.3389/fmicb.2015.00223},
  volume       = {6},
  year         = {2015},
}