Advanced

Bio-based 3-hydroxypropionic- and acrylic acid production from biodiesel glycerol via integrated microbial and chemical catalysis

Dishisha, Tarek LU ; Pyo, Sang-Hyun LU and Hatti-Kaul, Rajni LU (2015) In Microbial Cell Factories 14.
Abstract
Background

3-Hydroxypropionic acid (3HP) and acrylic acid (AA) are industrially important platform- and secondary chemical, respectively. Their production from renewable resources by environment-friendly processes is desirable. In the present study, both chemicals were almost quantitatively produced from biodiesel-derived glycerol by an integrated process involving microbial and chemical catalysis.



Results

Glycerol was initially converted in a fed-batch mode of operation to equimolar quantities of 3HP and 1,3-propanediol (1,3PDO) under anaerobic conditions using resting cells of Lactobacillus reuteri as a biocatalyst. The feeding rate of glycerol was controlled at 62.5 mg/gCDW.h which is half the... (More)
Background

3-Hydroxypropionic acid (3HP) and acrylic acid (AA) are industrially important platform- and secondary chemical, respectively. Their production from renewable resources by environment-friendly processes is desirable. In the present study, both chemicals were almost quantitatively produced from biodiesel-derived glycerol by an integrated process involving microbial and chemical catalysis.



Results

Glycerol was initially converted in a fed-batch mode of operation to equimolar quantities of 3HP and 1,3-propanediol (1,3PDO) under anaerobic conditions using resting cells of Lactobacillus reuteri as a biocatalyst. The feeding rate of glycerol was controlled at 62.5 mg/gCDW.h which is half the maximum metabolic flux of glycerol to 3HP and 1,3PDO through the L. reuteri propanediol-utilization (pdu) pathway to prevent accumulation of the inhibitory intermediate, 3-hydroxypronionaldehyde (3HPA). Subsequently, the cell-free supernatant containing the mixture of 3HP and 1,3PDO was subjected to selective oxidation under aerobic conditions using resting cells of Gluconobacter oxydans where 1,3PDO was quantitatively converted to 3HP in a batch system. The optimum conditions for the bioconversion were 10 g/L substrate and 5.2 g/L cell dry weight. Higher substrate concentrations led to enzyme inhibition and incomplete conversion. The resulting solution of 3HP was dehydrated to AA over titanium dioxide (TiO2) at 230 °C with a yield of >95 %.



Conclusions

The present study represents the first report on an integrated process for production of acrylic acid at high purity and -yield from glycerol through 3HP as intermediate without any purification step. The proposed process could have potential for industrial production of 3HP and AA after further optimization. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biocatalysis Cascade enzymatic reaction Lactobacillus reuteri Gluconobacter oxydans Catalytic dehydration Titanium dioxide 3-Hydroxypropionic acid Acrylic acid 1, 3-Propanediol Biodiesel glycerol
in
Microbial Cell Factories
volume
14
publisher
BioMed Central
external identifiers
  • pmid:26690945
  • wos:000367047300001
  • scopus:84954170297
ISSN
1475-2859
DOI
10.1186/s12934-015-0388-0
language
English
LU publication?
yes
id
4e136f0c-01b5-4d9b-bedd-9b102a4ad4a8 (old id 8508837)
date added to LUP
2016-01-07 12:51:51
date last changed
2017-11-19 03:45:20
@article{4e136f0c-01b5-4d9b-bedd-9b102a4ad4a8,
  abstract     = {Background<br/><br>
3-Hydroxypropionic acid (3HP) and acrylic acid (AA) are industrially important platform- and secondary chemical, respectively. Their production from renewable resources by environment-friendly processes is desirable. In the present study, both chemicals were almost quantitatively produced from biodiesel-derived glycerol by an integrated process involving microbial and chemical catalysis.<br/><br>
<br/><br>
Results<br/><br>
Glycerol was initially converted in a fed-batch mode of operation to equimolar quantities of 3HP and 1,3-propanediol (1,3PDO) under anaerobic conditions using resting cells of Lactobacillus reuteri as a biocatalyst. The feeding rate of glycerol was controlled at 62.5 mg/gCDW.h which is half the maximum metabolic flux of glycerol to 3HP and 1,3PDO through the L. reuteri propanediol-utilization (pdu) pathway to prevent accumulation of the inhibitory intermediate, 3-hydroxypronionaldehyde (3HPA). Subsequently, the cell-free supernatant containing the mixture of 3HP and 1,3PDO was subjected to selective oxidation under aerobic conditions using resting cells of Gluconobacter oxydans where 1,3PDO was quantitatively converted to 3HP in a batch system. The optimum conditions for the bioconversion were 10 g/L substrate and 5.2 g/L cell dry weight. Higher substrate concentrations led to enzyme inhibition and incomplete conversion. The resulting solution of 3HP was dehydrated to AA over titanium dioxide (TiO2) at 230 °C with a yield of &gt;95 %.<br/><br>
<br/><br>
Conclusions<br/><br>
The present study represents the first report on an integrated process for production of acrylic acid at high purity and -yield from glycerol through 3HP as intermediate without any purification step. The proposed process could have potential for industrial production of 3HP and AA after further optimization.},
  articleno    = {200},
  author       = {Dishisha, Tarek and Pyo, Sang-Hyun and Hatti-Kaul, Rajni},
  issn         = {1475-2859},
  keyword      = {Biocatalysis Cascade enzymatic reaction Lactobacillus reuteri Gluconobacter oxydans Catalytic dehydration Titanium dioxide 3-Hydroxypropionic acid Acrylic acid 1,3-Propanediol Biodiesel glycerol},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Microbial Cell Factories},
  title        = {Bio-based 3-hydroxypropionic- and acrylic acid production from biodiesel glycerol via integrated microbial and chemical catalysis},
  url          = {http://dx.doi.org/10.1186/s12934-015-0388-0},
  volume       = {14},
  year         = {2015},
}