Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Amylase intrapancreatic infusion delays insulin release during an intravenous glucose tolerance test, proof of acini–islet–acinar interactions

Pierzynowska, Kateryna LU orcid ; Wychowański, Piotr ; Zaworski, Kamil LU ; Woliński, Jarosław LU ; Donaldson, Janine ; Szkopek, Dominika ; Roszkowicz-Ostrowska, Katarzyna ; Kondej, Agata and Pierzynowski, Stefan G. LU (2024) In World Journal of Experimental Medicine 14(3).
Abstract

BACKGROUND The possible existence of an acini–islet–acinar (AIA) reflex, involving mutual amylase and insulin interactions, was investigated in the current acute experiment on pigs. AIM To confirm the existence of an AIA reflex and justify the placement of the exocrine and endocrine pancreatic components within the same organ. METHODS The study was performed on six pigs under general anesthesia. An intravenous glucose tolerance test was performed, with a bolus infusion of 50% glucose to the jugular vein, while amylase (5000 U/kg) or vehicle intrapancreatic infusions were administered via the pancreaticoduodenalis cranialis artery during 30 min with a 1 mL/min flow rate. RESULTS The amylase infusion to pancreatic arterial circulation... (More)

BACKGROUND The possible existence of an acini–islet–acinar (AIA) reflex, involving mutual amylase and insulin interactions, was investigated in the current acute experiment on pigs. AIM To confirm the existence of an AIA reflex and justify the placement of the exocrine and endocrine pancreatic components within the same organ. METHODS The study was performed on six pigs under general anesthesia. An intravenous glucose tolerance test was performed, with a bolus infusion of 50% glucose to the jugular vein, while amylase (5000 U/kg) or vehicle intrapancreatic infusions were administered via the pancreaticoduodenalis cranialis artery during 30 min with a 1 mL/min flow rate. RESULTS The amylase infusion to pancreatic arterial circulation inhibited and delayed the insulin release peak which is usually associated with the highest value of blood glucose and is typically observed at 15 min after glucose infusion, for > 1 h. The intrapancreatic infusion of the vehicle (saline) did not have any effect on the time frame of insulin release. Infusion of 1% bovine serum albumin changed the insulin release curve dramatically and prolonged the high range of insulin secretion, far beyond the glucose peak. CONCLUSION Intrapancreatic arterial infusion of amylase interrupted the integrated glucose–insulin interactions. This confirms an AIA reflex and justifies placement of the exocrine and endocrine pancreatic components within the same organ.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acini-islet-acinar axis, Amylase, Glucose-insulin-amylase interaction, Insulin, Intravenous glucose tolerance test
in
World Journal of Experimental Medicine
volume
14
issue
3
article number
92589
publisher
Baishideng Publishing Group
external identifiers
  • scopus:85202899889
  • pmid:39312707
DOI
10.5493/wjem.v14.i3.92589
language
English
LU publication?
yes
id
85436333-af25-439f-9384-dc133361b863
date added to LUP
2024-11-15 12:19:31
date last changed
2025-07-12 08:38:45
@article{85436333-af25-439f-9384-dc133361b863,
  abstract     = {{<p>BACKGROUND The possible existence of an acini–islet–acinar (AIA) reflex, involving mutual amylase and insulin interactions, was investigated in the current acute experiment on pigs. AIM To confirm the existence of an AIA reflex and justify the placement of the exocrine and endocrine pancreatic components within the same organ. METHODS The study was performed on six pigs under general anesthesia. An intravenous glucose tolerance test was performed, with a bolus infusion of 50% glucose to the jugular vein, while amylase (5000 U/kg) or vehicle intrapancreatic infusions were administered via the pancreaticoduodenalis cranialis artery during 30 min with a 1 mL/min flow rate. RESULTS The amylase infusion to pancreatic arterial circulation inhibited and delayed the insulin release peak which is usually associated with the highest value of blood glucose and is typically observed at 15 min after glucose infusion, for &gt; 1 h. The intrapancreatic infusion of the vehicle (saline) did not have any effect on the time frame of insulin release. Infusion of 1% bovine serum albumin changed the insulin release curve dramatically and prolonged the high range of insulin secretion, far beyond the glucose peak. CONCLUSION Intrapancreatic arterial infusion of amylase interrupted the integrated glucose–insulin interactions. This confirms an AIA reflex and justifies placement of the exocrine and endocrine pancreatic components within the same organ.</p>}},
  author       = {{Pierzynowska, Kateryna and Wychowański, Piotr and Zaworski, Kamil and Woliński, Jarosław and Donaldson, Janine and Szkopek, Dominika and Roszkowicz-Ostrowska, Katarzyna and Kondej, Agata and Pierzynowski, Stefan G.}},
  keywords     = {{Acini-islet-acinar axis; Amylase; Glucose-insulin-amylase interaction; Insulin; Intravenous glucose tolerance test}},
  language     = {{eng}},
  number       = {{3}},
  publisher    = {{Baishideng Publishing Group}},
  series       = {{World Journal of Experimental Medicine}},
  title        = {{Amylase intrapancreatic infusion delays insulin release during an intravenous glucose tolerance test, proof of acini–islet–acinar interactions}},
  url          = {{http://dx.doi.org/10.5493/wjem.v14.i3.92589}},
  doi          = {{10.5493/wjem.v14.i3.92589}},
  volume       = {{14}},
  year         = {{2024}},
}