Photoconjugation of temperature- and pH-responsive polymer with silica nanoparticles for separation and enrichment of bacteria
Zheng, Hongwei LU ; Gong, Haiyue LU ; Cao, Limin ; Lin, Hong and Ye, Lei LU
(2021)
In Colloids and Surfaces B: Biointerfaces
197.
- Abstract
A new photoconjugation approach was developed to prepare nanoparticle-supported boronic acid polymer for effective separation and enrichment of bacteria. The photo-activated polymer immobilization was demonstrated by coupling an azide-modified copolymer of N-isopropylacrylamide and glycidyl methacrylate to a perfluorophenyl azide-modified silica surface. The thermoresponsive polymer was synthesized using reversible addition fragmentation chain transfer polymerization followed by conversion of the pendant epoxides into azide groups. The perfluorophenyl azide-modified silica nanoparticles were synthesized by an amidation reaction between amino-functionalized silica and pentafluorobenzoyl chloride, and a subsequent treatment with sodium... (More)
A new photoconjugation approach was developed to prepare nanoparticle-supported boronic acid polymer for effective separation and enrichment of bacteria. The photo-activated polymer immobilization was demonstrated by coupling an azide-modified copolymer of N-isopropylacrylamide and glycidyl methacrylate to a perfluorophenyl azide-modified silica surface. The thermoresponsive polymer was synthesized using reversible addition fragmentation chain transfer polymerization followed by conversion of the pendant epoxides into azide groups. The perfluorophenyl azide-modified silica nanoparticles were synthesized by an amidation reaction between amino-functionalized silica and pentafluorobenzoyl chloride, and a subsequent treatment with sodium azide. Bacteria-capturing boronic acid was conjugated to the silica-supported polymer chains via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The particle size, morphology and organic content of the composite nanoparticles were characterized systematically. The capability of the nanocomposite to bind Gram-positive and Gram-negative bacteria was investigated. The nanocomposite exhibited high binding capacities for E. coli (13.4 × 107 CFU/mg) and S. epidermidis (7.66 × 107 CFU/mg) in phosphate buffered saline. The new photoconjugation strategy enables fast and straightforward grafting of functional polymers on surface, which opens many new opportunities for designing functional materials for bioseparation and biosensing.
(Less)
- author
- Zheng, Hongwei
LU
; Gong, Haiyue
LU
; Cao, Limin
; Lin, Hong
and Ye, Lei
LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacteria, Bioseparation, Boronate affinity, Perfluorophenyl azide, Photoconjugation, Polymer, Reversible addition fragmentation chain transfer polymerization
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 197
- article number
- 111433
- publisher
- Elsevier
- external identifiers
-
- scopus:85096242211
- pmid:33171436
- ISSN
- 0927-7765
- DOI
- 10.1016/j.colsurfb.2020.111433
- language
- English
- LU publication?
- yes
- id
- 8548bc76-28c8-417e-984f-b2e756bfb893
- date added to LUP
- 2020-11-30 09:37:13
- date last changed
- 2024-06-14 03:43:23
@article{8548bc76-28c8-417e-984f-b2e756bfb893,
abstract = {{<p>A new photoconjugation approach was developed to prepare nanoparticle-supported boronic acid polymer for effective separation and enrichment of bacteria. The photo-activated polymer immobilization was demonstrated by coupling an azide-modified copolymer of N-isopropylacrylamide and glycidyl methacrylate to a perfluorophenyl azide-modified silica surface. The thermoresponsive polymer was synthesized using reversible addition fragmentation chain transfer polymerization followed by conversion of the pendant epoxides into azide groups. The perfluorophenyl azide-modified silica nanoparticles were synthesized by an amidation reaction between amino-functionalized silica and pentafluorobenzoyl chloride, and a subsequent treatment with sodium azide. Bacteria-capturing boronic acid was conjugated to the silica-supported polymer chains via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The particle size, morphology and organic content of the composite nanoparticles were characterized systematically. The capability of the nanocomposite to bind Gram-positive and Gram-negative bacteria was investigated. The nanocomposite exhibited high binding capacities for E. coli (13.4 × 10<sup>7</sup> CFU/mg) and S. epidermidis (7.66 × 10<sup>7</sup> CFU/mg) in phosphate buffered saline. The new photoconjugation strategy enables fast and straightforward grafting of functional polymers on surface, which opens many new opportunities for designing functional materials for bioseparation and biosensing.</p>}},
author = {{Zheng, Hongwei and Gong, Haiyue and Cao, Limin and Lin, Hong and Ye, Lei}},
issn = {{0927-7765}},
keywords = {{Bacteria; Bioseparation; Boronate affinity; Perfluorophenyl azide; Photoconjugation; Polymer; Reversible addition fragmentation chain transfer polymerization}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Photoconjugation of temperature- and pH-responsive polymer with silica nanoparticles for separation and enrichment of bacteria}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2020.111433}},
doi = {{10.1016/j.colsurfb.2020.111433}},
volume = {{197}},
year = {{2021}},
}