Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245.
(2016) In Journal of Biotechnology 221. p.62-69- Abstract
- Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was... (More)
- Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1v/v/m airflow and 500rpm stirring speed. In batch mode of operation, 70.6% of 5g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep(®)900 (OH(-)), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8573895
- author
- Sayed, Mahmoud LU ; Dishisha, Tarek ; Sayed, Waiel F ; Salem, Wesam M A ; Temerk, Hanan A and Pyo, Sang-Hyun LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 221
- pages
- 62 - 69
- publisher
- Elsevier
- external identifiers
-
- pmid:26804932
- scopus:84956768841
- wos:000371459300009
- pmid:26804932
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2016.01.022
- language
- English
- LU publication?
- yes
- id
- f5094097-ea33-4bcd-af81-60fcfc08a9c5 (old id 8573895)
- date added to LUP
- 2016-04-01 10:25:49
- date last changed
- 2024-07-14 19:20:51
@article{f5094097-ea33-4bcd-af81-60fcfc08a9c5, abstract = {{Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl)butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5:5:2 was found optimum. The optimal conditions for biotransformation were pH 8, 1v/v/m airflow and 500rpm stirring speed. In batch mode of operation, 70.6% of 5g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep(®)900 (OH(-)), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation.}}, author = {{Sayed, Mahmoud and Dishisha, Tarek and Sayed, Waiel F and Salem, Wesam M A and Temerk, Hanan A and Pyo, Sang-Hyun}}, issn = {{1873-4863}}, language = {{eng}}, pages = {{62--69}}, publisher = {{Elsevier}}, series = {{Journal of Biotechnology}}, title = {{Selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using growing cells of Corynebacterium sp. ATCC 21245.}}, url = {{http://dx.doi.org/10.1016/j.jbiotec.2016.01.022}}, doi = {{10.1016/j.jbiotec.2016.01.022}}, volume = {{221}}, year = {{2016}}, }