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Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).

Ratanasopa, Khuanpiroon LU ; Cedervall, Tommy LU and Bülow, Leif LU (2016) In Advances in Experimental Medicine and Biology 876. p.445-453
Abstract
The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinant production processes, both for HbA and HbF, although the degree of heme oriental disorder is much lower for HbF. In addition, the heme disorientation can be converted to a normal conformation by an oxidation-reduction process. A chromatographic cleaning process involving a strong anion exchanger can be utilized to remove such unstable and nondesirable forms of Hb.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Advances in Experimental Medicine and Biology
volume
876
pages
445 - 453
publisher
Kluwer
external identifiers
  • pmid:26782244
  • scopus:84958554669
  • wos:000373880600057
ISSN
0065-2598
DOI
10.1007/978-1-4939-3023-4_56
language
English
LU publication?
yes
id
08b9eb1a-51af-400e-ad73-f0b12578d2ff (old id 8577081)
date added to LUP
2016-02-10 14:24:22
date last changed
2017-09-24 03:55:34
@article{08b9eb1a-51af-400e-ad73-f0b12578d2ff,
  abstract     = {The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinant production processes, both for HbA and HbF, although the degree of heme oriental disorder is much lower for HbF. In addition, the heme disorientation can be converted to a normal conformation by an oxidation-reduction process. A chromatographic cleaning process involving a strong anion exchanger can be utilized to remove such unstable and nondesirable forms of Hb.},
  author       = {Ratanasopa, Khuanpiroon and Cedervall, Tommy and Bülow, Leif},
  issn         = {0065-2598},
  language     = {eng},
  pages        = {445--453},
  publisher    = {Kluwer},
  series       = {Advances in Experimental Medicine and Biology},
  title        = {Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3023-4_56},
  volume       = {876},
  year         = {2016},
}