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Fast, rugged and sensitive ultra high pressure liquid chromatography tandem mass spectrometry method for analysis of cyanotoxins in raw water and drinking water-First findings of anatoxins, cylindrospermopsins and microcystin variants in Swedish source waters and infiltration ponds.

Pekar, Heidi; Westerberg, Erik; Bruno, Oscar; Lääne, Ants; Persson, Kenneth M LU ; Sundström, L Fredrik and Thim, Anna-Maria (2016) In Journal of chromatography. A 1429. p.265-276
Abstract
Freshwater blooms of cyanobacteria (blue-green algae) in source waters are generally composed of several different strains with the capability to produce a variety of toxins. The major exposure routes for humans are direct contact with recreational waters and ingestion of drinking water not efficiently treated. The ultra high pressure liquid chromatography tandem mass spectrometry based analytical method presented here allows simultaneous analysis of 22 cyanotoxins from different toxin groups, including anatoxins, cylindrospermopsins, nodularin and microcystins in raw water and drinking water. The use of reference standards enables correct identification of toxins as well as precision of the quantification and due to matrix effects,... (More)
Freshwater blooms of cyanobacteria (blue-green algae) in source waters are generally composed of several different strains with the capability to produce a variety of toxins. The major exposure routes for humans are direct contact with recreational waters and ingestion of drinking water not efficiently treated. The ultra high pressure liquid chromatography tandem mass spectrometry based analytical method presented here allows simultaneous analysis of 22 cyanotoxins from different toxin groups, including anatoxins, cylindrospermopsins, nodularin and microcystins in raw water and drinking water. The use of reference standards enables correct identification of toxins as well as precision of the quantification and due to matrix effects, recovery correction is required. The multi-toxin group method presented here, does not compromise sensitivity, despite the large number of analytes. The limit of quantification was set to 0.1μg/L for 75% of the cyanotoxins in drinking water and 0.5μg/L for all cyanotoxins in raw water, which is compliant with the WHO guidance value for microcystin-LR. The matrix effects experienced during analysis were reasonable for most analytes, considering the large volume injected into the mass spectrometer. The time of analysis, including lysing of cell bound toxins, is less than three hours. Furthermore, the method was tested in Swedish source waters and infiltration ponds resulting in evidence of presence of anatoxin, homo-anatoxin, cylindrospermopsin and several variants of microcystins for the first time in Sweden, proving its usefulness. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
1429
pages
265 - 276
publisher
Elsevier
external identifiers
  • pmid:26755412
  • scopus:84965168490
  • wos:000369559100029
ISSN
1873-3778
DOI
10.1016/j.chroma.2015.12.049
language
English
LU publication?
yes
id
649600d4-797c-493b-a45a-83d85713de86 (old id 8592355)
date added to LUP
2016-02-17 15:00:20
date last changed
2017-10-22 04:25:41
@article{649600d4-797c-493b-a45a-83d85713de86,
  abstract     = {Freshwater blooms of cyanobacteria (blue-green algae) in source waters are generally composed of several different strains with the capability to produce a variety of toxins. The major exposure routes for humans are direct contact with recreational waters and ingestion of drinking water not efficiently treated. The ultra high pressure liquid chromatography tandem mass spectrometry based analytical method presented here allows simultaneous analysis of 22 cyanotoxins from different toxin groups, including anatoxins, cylindrospermopsins, nodularin and microcystins in raw water and drinking water. The use of reference standards enables correct identification of toxins as well as precision of the quantification and due to matrix effects, recovery correction is required. The multi-toxin group method presented here, does not compromise sensitivity, despite the large number of analytes. The limit of quantification was set to 0.1μg/L for 75% of the cyanotoxins in drinking water and 0.5μg/L for all cyanotoxins in raw water, which is compliant with the WHO guidance value for microcystin-LR. The matrix effects experienced during analysis were reasonable for most analytes, considering the large volume injected into the mass spectrometer. The time of analysis, including lysing of cell bound toxins, is less than three hours. Furthermore, the method was tested in Swedish source waters and infiltration ponds resulting in evidence of presence of anatoxin, homo-anatoxin, cylindrospermopsin and several variants of microcystins for the first time in Sweden, proving its usefulness.},
  author       = {Pekar, Heidi and Westerberg, Erik and Bruno, Oscar and Lääne, Ants and Persson, Kenneth M and Sundström, L Fredrik and Thim, Anna-Maria},
  issn         = {1873-3778},
  language     = {eng},
  pages        = {265--276},
  publisher    = {Elsevier},
  series       = {Journal of chromatography. A},
  title        = {Fast, rugged and sensitive ultra high pressure liquid chromatography tandem mass spectrometry method for analysis of cyanotoxins in raw water and drinking water-First findings of anatoxins, cylindrospermopsins and microcystin variants in Swedish source waters and infiltration ponds.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2015.12.049},
  volume       = {1429},
  year         = {2016},
}