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Time-resolved total reflectance FT-IR spectroscopy characterization on conformational change of silk fibroin induced by acetic acid

Mo, Chun Li ; Dicko, Cedric LU ; Shao, Zheng Zhong and Chen, Xin (2009) In Acta Chimica Sinica 67(22). p.2641-2644
Abstract

It has been proved that time-resolved total reflectance FT-IR spectroscopy is a useful tool for investigating acetic acid diffusion in silk fibroin film and conformational transition of silk fibroin. Acetic acid could induce the construction of highly dominating content of β-sheet conformation in the silk fibroin film, in addition, the kinetic curve of organic solvent diffusion was similar to that of conformational transition as both processes reached equilibria at the same time. The access of acetic acid into the silk fibroin architecture appeared to be a necessary step in which the organic solvent could interact with silk protein, breaking its original hydrogen bond and inducing re-alignment of peptide chains. Furthermore, the content... (More)

It has been proved that time-resolved total reflectance FT-IR spectroscopy is a useful tool for investigating acetic acid diffusion in silk fibroin film and conformational transition of silk fibroin. Acetic acid could induce the construction of highly dominating content of β-sheet conformation in the silk fibroin film, in addition, the kinetic curve of organic solvent diffusion was similar to that of conformational transition as both processes reached equilibria at the same time. The access of acetic acid into the silk fibroin architecture appeared to be a necessary step in which the organic solvent could interact with silk protein, breaking its original hydrogen bond and inducing re-alignment of peptide chains. Furthermore, the content of water appeared to be an important factor in this dynamic system.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
β-sheet, Acidity-basicity, Diffusion, Dynamic process, Silk fibroin
in
Acta Chimica Sinica
volume
67
issue
22
pages
4 pages
publisher
Science Press
external identifiers
  • scopus:84884697717
ISSN
0567-7351
language
English
LU publication?
no
id
85a50bf7-84ef-4cf8-a520-d527ae504ca0
date added to LUP
2020-06-11 09:57:10
date last changed
2020-06-29 14:42:57
@article{85a50bf7-84ef-4cf8-a520-d527ae504ca0,
  abstract     = {<p>It has been proved that time-resolved total reflectance FT-IR spectroscopy is a useful tool for investigating acetic acid diffusion in silk fibroin film and conformational transition of silk fibroin. Acetic acid could induce the construction of highly dominating content of β-sheet conformation in the silk fibroin film, in addition, the kinetic curve of organic solvent diffusion was similar to that of conformational transition as both processes reached equilibria at the same time. The access of acetic acid into the silk fibroin architecture appeared to be a necessary step in which the organic solvent could interact with silk protein, breaking its original hydrogen bond and inducing re-alignment of peptide chains. Furthermore, the content of water appeared to be an important factor in this dynamic system.</p>},
  author       = {Mo, Chun Li and Dicko, Cedric and Shao, Zheng Zhong and Chen, Xin},
  issn         = {0567-7351},
  language     = {eng},
  month        = {11},
  number       = {22},
  pages        = {2641--2644},
  publisher    = {Science Press},
  series       = {Acta Chimica Sinica},
  title        = {Time-resolved total reflectance FT-IR spectroscopy characterization on conformational change of silk fibroin induced by acetic acid},
  volume       = {67},
  year         = {2009},
}