Ausencia de circulación de poliovirus en departamentos colombianos con coberturas vacunales inferiores a 80%
(2012) In Revista panamericana de salud publica = Pan American journal of public health 32(2). p.60-156- Abstract
This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio.... (More)
This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.
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- author
- González, María Mercedes ; Sarmiento, Luis LU ; Rey-Benito, Gloria Janneth ; Padilla, Leonardo ; Giraldo, Alejandra María and Castaño, Jhon Carlos
- alternative title
- Absence of poliovirus circulation in Colombian departments with vaccination coverage below 80%
- publishing date
- 2012-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Cell Line, Colombia, Enterovirus/classification, Humans, Mice, Poliovirus/classification, Poliovirus Vaccine, Oral, Serotyping, Vaccination/statistics & numerical data, Virus Cultivation, Waste Water/virology
- in
- Revista panamericana de salud publica = Pan American journal of public health
- volume
- 32
- issue
- 2
- pages
- 5 pages
- publisher
- Pan American Health Organization
- external identifiers
-
- pmid:23099878
- scopus:84868012885
- ISSN
- 1020-4989
- DOI
- 10.1590/s1020-49892012000800011
- language
- Spanish
- LU publication?
- no
- id
- 85a8e718-43a1-423c-8f4c-f068ca480928
- date added to LUP
- 2019-07-08 14:36:30
- date last changed
- 2024-01-04 22:37:20
@article{85a8e718-43a1-423c-8f4c-f068ca480928,
abstract = {{<p>This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.</p>}},
author = {{González, María Mercedes and Sarmiento, Luis and Rey-Benito, Gloria Janneth and Padilla, Leonardo and Giraldo, Alejandra María and Castaño, Jhon Carlos}},
issn = {{1020-4989}},
keywords = {{Animals; Cell Line; Colombia; Enterovirus/classification; Humans; Mice; Poliovirus/classification; Poliovirus Vaccine, Oral; Serotyping; Vaccination/statistics & numerical data; Virus Cultivation; Waste Water/virology}},
language = {{spa}},
number = {{2}},
pages = {{60--156}},
publisher = {{Pan American Health Organization}},
series = {{Revista panamericana de salud publica = Pan American journal of public health}},
title = {{Ausencia de circulación de poliovirus en departamentos colombianos con coberturas vacunales inferiores a 80%}},
url = {{http://dx.doi.org/10.1590/s1020-49892012000800011}},
doi = {{10.1590/s1020-49892012000800011}},
volume = {{32}},
year = {{2012}},
}