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Ausencia de circulación de poliovirus en departamentos colombianos con coberturas vacunales inferiores a 80%

González, María Mercedes ; Sarmiento, Luis LU ; Rey-Benito, Gloria Janneth ; Padilla, Leonardo ; Giraldo, Alejandra María and Castaño, Jhon Carlos (2012) In Revista panamericana de salud publica = Pan American journal of public health 32(2). p.60-156
Abstract

This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio.... (More)

This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.

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author
alternative title
Absence of poliovirus circulation in Colombian departments with vaccination coverage below 80%
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Cell Line, Colombia, Enterovirus/classification, Humans, Mice, Poliovirus/classification, Poliovirus Vaccine, Oral, Serotyping, Vaccination/statistics & numerical data, Virus Cultivation, Waste Water/virology
in
Revista panamericana de salud publica = Pan American journal of public health
volume
32
issue
2
pages
5 pages
publisher
Pan American Health Organization
external identifiers
  • pmid:23099878
ISSN
1020-4989
DOI
10.1590/s1020-49892012000800011
language
Spanish
LU publication?
no
id
85a8e718-43a1-423c-8f4c-f068ca480928
date added to LUP
2019-07-08 14:36:30
date last changed
2019-10-08 04:01:03
@article{85a8e718-43a1-423c-8f4c-f068ca480928,
  abstract     = {<p>This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.</p>},
  author       = {González, María Mercedes and Sarmiento, Luis and Rey-Benito, Gloria Janneth and Padilla, Leonardo and Giraldo, Alejandra María and Castaño, Jhon Carlos},
  issn         = {1020-4989},
  language     = {spa},
  number       = {2},
  pages        = {60--156},
  publisher    = {Pan American Health Organization},
  series       = {Revista panamericana de salud publica = Pan American journal of public health},
  title        = {Ausencia de circulación de poliovirus en departamentos colombianos con coberturas vacunales inferiores a 80%},
  url          = {http://dx.doi.org/10.1590/s1020-49892012000800011},
  doi          = {10.1590/s1020-49892012000800011},
  volume       = {32},
  year         = {2012},
}