Nanohybrid polymer brushes on silica for bioseparation
Jiang, Lingdong LU ; Bagan Navarro, Hector LU ; Kamra, Tripta LU ; Zhou, Tongchang LU and Ye, Lei LU
(2016)
In Journal of Materials Chemistry. B
4(19).
p.3247-3256
- Abstract
Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier... (More)
Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm2. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g-1 and 22.8 mg g-1, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.
(Less)
- author
- Jiang, Lingdong
LU
; Bagan Navarro, Hector
LU
; Kamra, Tripta
LU
; Zhou, Tongchang
LU
and Ye, Lei
LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Materials Chemistry. B
- volume
- 4
- issue
- 19
- pages
- 10 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000376039100009
- scopus:84969964561
- ISSN
- 2050-7518
- DOI
- 10.1039/c6tb00241b
- language
- English
- LU publication?
- yes
- id
- 85d206f3-1231-40bc-9d3d-cd0075240f14
- date added to LUP
- 2016-07-19 07:50:04
- date last changed
- 2024-03-22 05:29:38
@article{85d206f3-1231-40bc-9d3d-cd0075240f14,
abstract = {{<p>Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm<sup>2</sup>. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g<sup>-1</sup> and 22.8 mg g<sup>-1</sup>, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.</p>}},
author = {{Jiang, Lingdong and Bagan Navarro, Hector and Kamra, Tripta and Zhou, Tongchang and Ye, Lei}},
issn = {{2050-7518}},
language = {{eng}},
number = {{19}},
pages = {{3247--3256}},
publisher = {{Royal Society of Chemistry}},
series = {{Journal of Materials Chemistry. B}},
title = {{Nanohybrid polymer brushes on silica for bioseparation}},
url = {{http://dx.doi.org/10.1039/c6tb00241b}},
doi = {{10.1039/c6tb00241b}},
volume = {{4}},
year = {{2016}},
}